Abstract
Fetal exposure to alcohol can cause a wide range of long-lasting physiological and behavioral effects, collectively referred to as fetal alcohol spectrum disorders (FASD). Neurocognitive disorders caused by alcohol in children are linked to activation of oxidative-inflammatory cascade and a high level of apoptotic neurodegeneration in several brain regions, such as hippocampus. NCB2/nesfatin-1 is a novel identified anorexigenic peptide. In addition recently, it has been reported as anti-apoptotic and anti-inflammatory properties of nesfatin-1 under both in vitro and in vivo situations. This research aimed at evaluating the protective activities of nesfatin-1 on ethanol-induced neuroinflammation and neuronal apoptosis in rat hippocampus with postnatal alcohol exposure. Administration of 5.27 g/kg of ethanol in milk solution 27.8 mL/kg to male rat pups was performed through intragastric intubation on postnatal days 2–10. The pups were administered 5 and 10 µg/kg of nesfatin-1 on postnatal days 2–10. For examining the spatial memory, Morris water maze test was carried out 36 days after birth. Following the behavioral test, immunohistochemical staining was performed to evaluate the expression levels of GFAP and apoptotic cell death was detected by TUNEL staining, also ELISA assay was done for measuring TNF-α, and antioxidant enzymes levels. Nesfatin-1 treatment could significantly improve spatial memory impairment (P < 0.01). Also it could significantly increase the activity of total SOD and GSH-Px (P < 0.05). Nesfatin-1 treatment caused a decrease in TNF-α and MDA levels, than the ethanol group (P < 0.01). Also it reduced the hippocampal apoptotic cells death, and GFAP-positive cells (P < 0.01). Based on the findings, apoptotic signaling mediated by the oxidative-inflammatory cascade is inhibited by nesfatin-1 in rat pups with ethanol exposure in the postnatal period.
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References
Abbott CW, Rohac DJ, Bottom RT, Patadia S, Huffman KJ (2017) Prenatal ethanol exposure and neocortical development: a transgenerational model of Fasd. Cereb Cortex 28:2908–2921
Amin FU, Shah SA, Kim MO (2016) Glycine inhibits ethanol-induced oxidative stress, neuroinflammation and apoptotic neurodegeneration in postnatal rat brain. Neurochem Int 96:1–12
Aski ML, Rezvani ME, Khaksari M, Hafizi Z, Pirmoradi Z, Niknazar S, Mehrjerdi FZ (2018) Neuroprotective effect of berberine chloride on cognitive impairment and hippocampal damage in experimental model of vascular dementia. Iran J Basic Med Sci 21:53
Bélanger M, Allaman I, Magistretti PJ (2011) Brain energy metabolism: focus on astrocyte-neuron metabolic cooperation. Cell Metab 14:724–738
Bharath S, Hsu M, Kaur D, Rajagopalan S, Andersen JK (2002) Glutathione, iron and Parkinson’s disease. Biochem Pharmacol 64:1037–1048
Boitard C, Cavaroc A, Sauvant J, Aubert A, Castanon N, Layé S, Ferreira G (2014) Impairment of hippocampal-dependent memory induced by juvenile high-fat diet intake is associated with enhanced hippocampal inflammation in rats. Brain Behav Immun 40:9–17
Bonnet MS, Pecchi E, Trouslard J, Jean A, Dallaporta M, Troadec J-D (2009) Central Nesfatin-1-expressing neurons are sensitive to peripheral inflammatory stimulus. J Neuroinflamm 6:27
Boschen KE, Ruggiero MJ, Klintsova AY (2016) Neonatal binge alcohol exposure increases microglial activation in the developing rat hippocampus. Neuroscience 324:355–366
Brocardo PS, Gil-Mohapel J, Christie BR (2011) The role of oxidative stress in fetal alcohol spectrum disorders. Brain Res Rev 67:209–225
Brocardo PS, Gil-Mohapel J, Wortman R, Noonan A, Mcginnis E, Patten AR, Christie BR (2017) The effects of ethanol exposure during distinct periods of brain development on oxidative stress in the adult rat brain. Alcoholism 41:26–37
Byrnes M, Richardson D, Brien J, Reynolds J, Dringenberg H (2004) Spatial acquisition in the morris water maze and hippocampal long-term potentiation in the adult guinea pig following brain growth spurt-prenatal ethanol exposure. Neurotoxicol Teratol 26:543–551
Castellani RJ, Moreira PI, Liu G, Dobson J, Perry G, Smith MA, Zhu X (2007) Iron: the redox-active center of oxidative stress in alzheimer disease. Neurochem Res 32:1640–1645
Chastain LG, Sarkar DK (2014) Role of microglia in regulation of ethanol neurotoxic action. Int Rev Neurobiol 118:81–103
Colombo E, Farina C (2016) Astrocytes: key regulators of neuroinflammation. Trends Immunol 37:608–620
Erfani S, Moghimi A, Aboutaleb N, Khaksari M (2018) Nesfatin-1 improve spatial memory impairment following transient global cerebral ischemia/reperfusion via inhibiting microglial and caspase-3 activation. J Mol Neurosci 65:377–384
Erfani S, Moghimi A, Aboutaleb N, Khaksari M (2019) Protective effects of Nesfatin-1 peptide on cerebral ischemia reperfusion injury via inhibition of neuronal cell death and enhancement of antioxidant defenses. Metab Brain Dis 34:79–85
Ghanbari F, Khaksari M, Vaezi G, Hojati V, Shiravi A (2019) hydrogen sulfide protects hippocampal neurons against methamphetamine neurotoxicity via inhibition of apoptosis and neuroinflammation. J Mol Neurosci 67:133–141
Guerri C, Pascual M (2010) Mechanisms involved in the neurotoxic, cognitive, and neurobehavioral effects of alcohol consumption during adolescence. Alcohol 44:15–26
Guerri C, Bazinet A, Riley EP (2009) Foetal alcohol spectrum disorders and alterations in brain and behaviour. Alcohol Alcohol 44:108–114
Guizzetti M, Zhang X, Goeke C, Gavin DP (2014) Glia and neurodevelopment: focus on fetal alcohol spectrum disorders. Front Pediatr 2:123
Heaton MB, Paiva M, Madorsky I, Mayer J, Moore DB (2003) Effects of ethanol on neurotrophic factors, apoptosis-related proteins, endogenous antioxidants, and reactive oxygen species in neonatal striatum: relationship to periods of vulnerability. Dev Brain Res 140:237–252
Helfer JL, Goodlett CR, Greenough WT, Klintsova AY (2009) The effects of exercise on adolescent hippocampal neurogenesis in a rat model of binge alcohol exposure during the brain growth spurt. Brain Res 1294:1–11
Hol EM, Pekny M (2015) Glial fibrillary acidic protein (Gfap) and the astrocyte intermediate filament system in diseases of the central nervous system. Curr Opin Cell Biol 32:121–130
Joya X, Garcia-Algar O, Salat-Batlle J, Pujades C, Vall O (2015) Advances in the development of novel antioxidant therapies as an approach for fetal alcohol syndrome prevention. Birth Defects Res A 103:163–177
Kelly SJ, Goodlett CR, Hannigan JH (2009) Animal models of fetal alcohol spectrum disorders: impact of the social environment. Dev Disabil Res Rev 15:200–208
Kempuraj D, Thangavel R, Natteru P, Selvakumar G, Saeed D, Zahoor H, Zaheer S, Iyer S, Zaheer A (2016) Neuroinflammation induces neurodegeneration. J Neurol Neurosurg Spine 1:1003
Khaksari M, Mehrjerdi FZ, Rezvani ME, Safari F, Mirgalili A, Niknazar S (2017) The role of erythropoietin in remote renal preconditioning on hippocampus ischemia/reperfusion injury. J Physiol Sci 67:163–171
Kolgazi M, Cantali-Ozturk C, Deniz R, Ozdemir-Kumral ZN, Yuksel M, Sirvanci S, Yeğen BC (2015) Nesfatin-1 alleviates gastric damage via direct antioxidant mechanisms. J Surg Res 193:111–118
Komada M, Hara N, Kawachi S, Kawachi K, Kagawa N, Nagao T, Ikeda Y (2017) Mechanisms underlying neuro-inflammation and neurodevelopmental toxicity in the mouse neocortex following prenatal exposure to ethanol. Sci Rep 7:4934
Marino MD, Cronise K, Lugo JN Jr, Kelly SJ (2002) Ultrasonic vocalizations and maternal-infant interactions in a rat model of fetal alcohol syndrome. Dev Psychobiol 41:341–351
Mcgoey TN, Reynolds JN, Brien JF (2003) Chronic prenatal ethanol exposure-induced decrease of guinea pig hippocampal Ca1 pyramidal cell and cerebellar purkinje cell density. Can J Physiol Pharmacol 81:476–484
Mckenna MC, Dienel GA, Sonnewald U, Waagepetersen HS, Schousboe A (2012) Energy metabolism of the brain. Basic Neurochem 1:221
Moreno E, Yan M, Basler K (2002) Evolution of Tnf signaling mechanisms: Jnk-dependent apoptosis triggered by Eiger, the drosophila homolog of the Tnf superfamily. Curr Biol 12:1263–1268
Muralidharan P, Sarmah S, Zhou FC, Marrs JA (2013) Fetal alcohol spectrum disorder (Fasd) associated neural defects: complex mechanisms and potential therapeutic targets. Brain Sci 3:964–991
Nazarnezhad S, Rahmati M, Shayannia A, Abbasi Z, Salehi M, Khaksari M (2019) Nesfatin-1 protects Pc12 cells against high glucose-induced cytotoxicity via inhibiting oxidative stress, autophagy and apoptosis. Neurotoxicology 74:196–202
Özsavcí D, Erşahin M, Şener A, Özakpinar ÖB, Toklu HZ, Akakín D, Şener G, Yeğen BÇ (2011) The novel function of Nesfatin-1 as an anti-inflammatory and antiapoptotic peptide in subarachnoid hemorrhage-induced oxidative brain damage in rats. Neurosurgery 68:1699–1708
Pascual M, Blanco AM, Cauli O, Miñarro J, Guerri C (2007) Intermittent ethanol exposure induces inflammatory brain damage and causes long-term behavioural alterations in adolescent rats. Eur J Neurosci 25:541–550
Raghunathan R, Wu C, Singh M, Liu CH, Miranda RC, Larin KV (2018) Evaluating the effects of maternal alcohol consumption on murine fetal brain vasculature using optical coherence tomography. J Biophotonics 11:E201700238
Rasmussen C (2005) Executive functioning and working memory in fetal alcohol spectrum disorder. Alcoholism 29:1359–1367
Riley EP, Mcgee CL (2005) Fetal alcohol spectrum disorders: an overview with emphasis on changes in brain and behavior. Exp Biol Med 230:357–365
Saito M, Chakraborty G, Hui M, Masiello K, Saito M (2016) Ethanol-induced neurodegeneration and glial activation in the developing brain. Brain Sci 6:31
Shafahi M, Vaezi G, Shajiee H, Sharafi S, Khaksari M (2018) Crocin inhibits apoptosis and astrogliosis of hippocampus neurons against methamphetamine neurotoxicity via antioxidant and anti-inflammatory mechanisms. Neurochem Res 43:2252–2259
Shah SA, Yoon GH, Kim MO (2015) Protection of the developing brain with anthocyanins against ethanol-induced oxidative stress and neurodegeneration. Mol Neurobiol 51:1278–1291
Swart PC, Currin CB, Russell VA, Dimatelis JJ (2017) Early ethanol exposure and vinpocetine treatment alter learning-and memory-related proteins in the rat hippocampus and prefrontal cortex. J Neurosci Res 95:1204–1215
Szymonik-Lesiuk S, Czechowska G, Stryjecka-Zimmer M, Słomka M, Mąldro A, Celiński K, Wielosz M (2003) Catalase, superoxide dismutase, and glutathione peroxidase activities in various rat tissues after carbon tetrachloride intoxication. J Hepato-Biliary-Pancreat Surg 10:309–315
Tan Z, Xu H, Shen X, Jiang H (2015) Nesfatin-1 antagonized rotenone-induced neurotoxicity in Mes23. 5 Dopaminergic cells. Peptides 69:109–114
Tang C-H, Fu X-J, Xu X-L, Wei X-J, Pan H-S (2012) The anti-inflammatory and anti-apoptotic effects of Nesfatin-1 in the traumatic rat brain. Peptides 36:39–45
Toosi A, Shajiee H, Khaksari M, Vaezi G, Hojati V (2019) Obestatin improve spatial memory impairment in a rat model of fetal alcohol spectrum disorders via inhibiting apoptosis and neuroinflammation. Neuropeptides 74:88–94
Tran TD, Kelly SJ (2003) Critical periods for ethanol-induced cell loss in the hippocampal formation. Neurotoxicol Teratol 25:519–528
Vallés SL, Blanco AM, Pascual M, Guerri C (2004) Chronic ethanol treatment enhances inflammatory mediators and cell death in the brain and in astrocytes. Brain Pathol 14:365–371
West JR, Hamre KM, Pierce DR (1984) Delay in brain growth induced by alcohol in artificially reared rat pups. Alcohol 1:213–222
Zhang D, Hu X, Qian L, O’callaghan JP, Hong J-S, (2010) Astrogliosis in Cns pathologies: is there a role for microglia? Mol Neurobiol 41:232–241
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Ghamari, F., Vaezi, G., Khaksari, M. et al. Nesfatin-1 Ameliorate Learning and Memory Deficit via Inhibiting Apoptosis and Neuroinflammation Following Ethanol-Induced Neurotoxicity in Early Postnatal Rats. Int J Pept Res Ther 26, 2029–2038 (2020). https://doi.org/10.1007/s10989-019-09999-w
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DOI: https://doi.org/10.1007/s10989-019-09999-w