Abstract
Peptides test drug 1, 2, 3 and 4 coded as RK, KH, RRH, and RRK were subjected to in-vitro antioxidant screening methods for the assessment of their potency using Ascorbic acid as the standard drug. Antioxidant screening in-vitro methods used are such as Superoxide radical scavenging activity, Reducing power method, Phosphomolybdenum method, Ferric thiocyanate method, Thiobarbituric acid method, β-Carotene linoleic acid method/conjugated diene assay, and Fenton's reagent method. Different concentrations of test drugs were made and antioxidant activity was calculated by estimating inhibition percentage and IC50 values which were consequently compared with standard drug. The entire test drug RK, KH, RRH, and RRK showed relatively better antioxidant competence with that of the standard drug. Based on the above estimations, KH and RRH could be considered as the best potent drug for the diverse formulations of antioxidant effect suitable for the avoidance of various human diseases.
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References
Abdeldaiem MH (2014) Use of yellow pigment extracted from turmeric (Curcuma Longa) rhizomes powder as natural food preservative. Am J Food Sci Technol 1:36–47. https://doi.org/10.12691/ajfst-2-1-6
Abdulle AE, Diercks GFH, Feelisch M, Mulder DJ, Goor H (2018) The role of oxidative stress in the development of systemic sclerosis related vasculopathy. Front Physiol. https://doi.org/10.3389/fphys.2018.01177
Aiyengar TM, Chiranjeevi P, Rani HS (2017) Role of endothelial nitric oxide synthase in breast cancer. Nitric Oxide Synthase. https://doi.org/10.5772/67493
Barajas B, Che N, Yin F, Rowshanrad A, Orozco LD, Gong KW, Araujo JA (2010) NF-E2-related factor 2 promotes atherosclerosis by effects on plasma lipoproteins and cholesterol transport that overshadow antioxidant protection. Arterioscler Thromb Vasc Biol 31(1):58–66. https://doi.org/10.1161/ATVBAHA.110.210906
Cahill PA, Redmond EM (2016) Vascular endothelium – Gatekeeper of vessel health. Atherosclerosis 248:97–109. https://doi.org/10.1016/j.atherosclerosis.2016.03.007
Cicero AFG, Fogacci F, Colletti A (2016) Potential role of bioactive peptides in prevention and treatment of chronic diseases: a narrative review. Br J Pharmacol 174(11):1378–1394. https://doi.org/10.1111/bph.13608
Davies MJ (2016) Protein oxidation and peroxidation. Biochem J 473(7):805–825. https://doi.org/10.1042/BJ20151227
Díaz-Gómez JL, Castorena-Torres F, Preciado-Ortiz RE, García-Lara S (2017) Anti-cancer activity of maize bioactive peptides. Front Chem. https://doi.org/10.3389/fchem.2017.00044
Engwa GA (2018) Free Radicals and the role of plant phytochemicals as antioxidants against oxidative stress-related diseases. Phytochemicals. https://doi.org/10.5772/intechpen.76719
Gaunitz F, Hipkiss AR (2012) Carnosine and cancer: a perspective. Amino Acids 43(1):135–142. https://doi.org/10.1007/s00726-012-1271-5
He L, He T, Farrar S, Ji L, Liu T, Ma X (2017) Antioxidants maintain cellular redox homeostasis by elimination of reactive oxygen species. Cell Physiol Biochem 44(2):532–553. https://doi.org/10.1159/000485089
Ivanova EA, Myasoedova VA, Melnichenko AA, Grechko AV, Orekhov AN (2017) Small dense low-density lipoprotein as biomarker for atherosclerotic diseases. Oxid Med Cell Long 2017:1–10. https://doi.org/10.1155/2017/1273042
Jayaprakash GK, Singh RP, Sakrish KK (2001) Antioxidant activity of grapes seed extracts on peroxidation modles in-vitro. J Agric Food Chem 55:1018–1022. https://doi.org/10.1016/S0308-8146(00)00298-3
Kabouch A, Kabouch Z, Ozturk M, Kolal U, Topcu G (2007) Antioxidant abietane diterpenoids from Salvia barrelieri. Food Chem 102:1281–1287. https://doi.org/10.1002/pca.1130
Kara A, Gedikli S, Sengul E, Gelen V, Ozkanlar S (2016) Oxidative stress and autophagy. Free Rad Dis. https://doi.org/10.5772/64569
Kumari S, Badana AK, Murli MG, Shailendra G, Malla R (2018) Reactive oxygen species: a key constituent in cancer survival. Biomark Insights. https://doi.org/10.1177/1177271918755391
Kurutas EB (2015) The importance of antioxidants which play the role in cellular response against oxidative/nitrosative stress: current state. Nutr J. https://doi.org/10.1186/s12937-016-0186-5
Levine AS, Sun L, Tan R et al (2017) The oxidative DNA damage response: a review of research undertaken with Tsinghua and Xiangya students at the University of Pittsburgh. Sci China Life Sci 60:1077–1080. https://doi.org/10.1007/s11427-017-9184-6
Li J-K, Liu X-D, Shen L, Zeng W-M, Qiu G-Z (2016) Natural plant polyphenols for alleviating oxidative damage in man: current status and future perspectives. Trop J Pharm Res 15(5):1089. https://doi.org/10.4314/tjpr.v.15i5.27
Liguori I, Russo G, Curcio F, Bulli G, Aran L, Della-Morte D, Abete P (2018) Oxidative stress, aging, and diseases. Clin Interv Aging 13:757–772. https://doi.org/10.2147/CIA.S158513
Lv H, She G (2010) Naturally occurring diarylheptanoids. Nat Prod Commun. https://doi.org/10.1177/1934578X1000501035
Md NA, Nusrat JB, Md R (2013) Review on in-vivo and in-vitro methods evalution of antioxidant activity. Saudi Pharm J 21:14–152. https://doi.org/10.1016/j.jsps.2012.05.002
Meléndez-Martínez AJ, Stinco CM, Mapelli-Brahm P (2019) Skin carotenoids in public health and nutricosmetics: the emerging roles and applications of the uv radiation-absorbing colourless carotenoids phytoene and phytofluene. Nutrients 11(5):1093. https://doi.org/10.3390/nu11051093
Minero C, Lucchiari M, Maurino V, Vione D (2013) A quantitative assessment of the production of ˙OH and additional oxidants in the dark Fenton reaction: Fenton degradation of aromatic amines. RSC Adv 3(48):26443. https://doi.org/10.1039/c3ra44585b
Nagarajan K, Mazumdar A, Ghosh LK (2008) In-vitro antioxidant activity of alcholic extraxts of Wrightia tomentosa. Pharmacologyonline. 1:196–203. https://doi.org/10.7897/2277-4343.05118
Nita M, Grzybowski A (2016a) The role of the reactive oxygen species and oxidative stress in the pathomechanism of the age-related ocular diseases and other pathologies of the anterior and posterior eye segments in adults. Oxid Med Cell Longev 2016:1–23. https://doi.org/10.1159/000485089
Nita M, Grzybowski A (2016b) The role of the reactive oxygen species and oxidative stress in the pathomechanism of the age-related ocular diseases and other pathologies of the anterior and posterior eye segments in adults. Oxid Med Cell Longev. https://doi.org/10.1155/2016/3164734
Ogrodnik M, Salmonowicz H, Gladyshev VN (2018) Integrating cellular senescence with the concept of damage accumulation in aging: relevance for clearance of senescent cells. Aging Cell. https://doi.org/10.1111/acel.12841
Pisoschi AM, Negulescu GP (2012) Methods for total antioxidant activity determination: a review. Biochem Anal Biochem. https://doi.org/10.4172/2161-1009.1000106
Pisoschi AM, Pop A, Cimpeanu C, Predoi G (2016) Antioxidant capacity determination in plants and plant-derived products: a review. Oxid Med Cell Longev 2016:1–36. https://doi.org/10.1155/2016/9130976
Pizzino G, Irrera N, Cucinotta M, Pallio G, Mannino F, Arcoraci V, Bitto A (2017) Oxidative stress: harms and benefits for human health. Oxid Med Cell Longev. https://doi.org/10.1155/2017/8416763
Qian ZJ, Jung WK, Kim SK (2008) Free radical scavenging activity of novel antioxidative peptide purified from hydrolysate of bullfrog skin (Rana catesbeiana shaw). Bioresour Technol 99:1690–1698. https://doi.org/10.1016/j.biortech.2007.04.005
Sailaja Rao P, Kalva S, Yerramilli A, Mamidi S (2011) Free Radicals and tissue damage: role of antioxidants. Free Rad Antioxid 1(4):2–7. https://doi.org/10.5530/ax.2011.4.2
Saeed N, Khan MR, Shabbir M (2012) Antioxidant activity, total phenolic and total flavonoid contents of whole plant extracts Torilis leptophylla L. BMC Complem Altern Med. https://doi.org/10.1186/1472-6882-12-221
Sathasivam R, Ki JS (2018) A review of the biological activities of microalgal carotenoids and their potential use in healthcare and cosmetic industries. Mar Drugs 16(1):26. https://doi.org/10.3390/md16010026
Sharififar F, Dehghn-Nudeh G, Mirtajaldini M (2009) Major flavonoids with antioxidant activity from Teucrium polium L. Food Chem 112(4):885–888. https://doi.org/10.1016/j.foodchem.2008.06.064
Sochor J, Ryvolova M, Krystofova O, Salas P, Hubalek J et al (2010) Fully automated spectrometric protocols for determination of antioxidant activity: advantages and disadvantages. Molecules 15(12):8618–8640. https://doi.org/10.3390/molecules15128618
Tan BL, Norhaizan ME, Liew WP, Sulaiman Rahman H (2018) Antioxidant and oxidative stress: a mutual interplay in age-related diseases. Front Pharmacol. https://doi.org/10.3389/fphar.2018.01162
Wu Y, Wang J, Li L, Yang X, Wang J, Hu X (2017) Purification and identification of an antioxidant peptide from Pinctada fucata muscle. CyTA 16(1):11–19. https://doi.org/10.1080/19476337.2017.1332099
Ye ZW, Zhang J, Townsend DM (1850) Tew KD (2015) Oxidative stress, redox regulation and diseases of cellular differentiation. Biochim Biophys Acta 8:1607–1621. https://doi.org/10.1016/j.bbagen.2014.11.010
You L, Zhao M, Cui C, Zhao H, Yang B (2009) Effect of degree of hydrolysis on the antioxidant activity of loach (Misgurnus anguillicaudatus) protein hydrolysates. Innov Food Sci Emerg Technol 10:235–240. https://doi.org/10.1016/j/ifset.2008.08.007
Zhang W, Xiao S, Ahn DU (2013) Protein oxidation: basic principles and implications for meat quality. Crit Rev Food Sci Nutr 53(11):1191–1201. https://doi.org/10.1080/10408398.2011.577540
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Mishra, M., Nagarajan, K. An Insight for Potent In-Vitro Antioxidant Status of Short-Chain Peptides. Int J Pept Res Ther 26, 1437–1449 (2020). https://doi.org/10.1007/s10989-019-09945-w
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DOI: https://doi.org/10.1007/s10989-019-09945-w