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In Silico Designing a Candidate Vaccine Against Breast Cancer

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Abstract

Breast cancer (BC) is the most common type of women’s cancer with a prevalence of about 25%, although it is rare in men (< 1%). Until now, BC therapy approaches have not been successful for various reasons. The purpose of this study was to design a candidate vaccine against BC using bioinformatics tools. Herein, in silico design of a fusion vaccine candidate containing extracellular domain of HER-2/neu protein and N-terminal sequence of GP96 protein (NTGP96) were performed. Different bioinformatics and immunoinformatics softwares were used to study the important characteristics of the designed vaccine, including physicochemical properties, secondary, and 3D structures, as well as antigenicity and allergenicity. Structural and immunological computational results indicated the potential of our designed construct for proper stimulation of cellular and humoral immune responses against BC. According to the obtained data, the candidate vaccine could be introduced for BC therapy, after the efficacy of it was confirmed by in vivo and in vitro immunological assays.

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Acknowledgements

The authors wish to thank Shiraz University of Medical Sciences for supporting the conduct of this research.

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Authors and Affiliations

  1. Department of Medical Biotechnology, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran

    Amir Atapour, Younes Ghasemi & Amir Savardashtaki

  2. Pharmaceutical Sciences Research Center, Shiraz University of Medical Sciences, Shiraz, Iran

    Manica Negahdaripour, Younes Ghasemi, Seyyed Mojtaba Mousavi, Seyyed Alireza Hashemi & Navid Nezafat

  3. Department of Pharmaceutical Biotechnology, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran

    Manica Negahdaripour, Younes Ghasemi & Navid Nezafat

  4. Cardiac Surgery, Namazi Hospital, Shiraz University of Medical Sciences, Shiraz, Iran

    Dorna Razmjuee

  5. Department of Physiology, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran

    Ali Aliabadi

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  1. Amir Atapour
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  2. Manica Negahdaripour
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Atapour, A., Negahdaripour, M., Ghasemi, Y. et al. In Silico Designing a Candidate Vaccine Against Breast Cancer. Int J Pept Res Ther 26, 369–380 (2020). https://doi.org/10.1007/s10989-019-09843-1

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