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Synthesis, Characterization and Lipophilicity Study of Brucella abortus’ Immunogenic Peptide Sequence That Can Be Used in the Future Vaccination Studies

International Journal of Peptide Research and Therapeutics volume 25, pages 911–918 (2019)Cite this article

Abstract

Brucellosis is primarily a zoonotic disease caused by members of the Brucella genus which consists of 11 recognized species based on pathogenicity and host preferences. Peptide vaccines are an alternative strategy to conventional vaccines based on the use of short peptide sequences and engineered to induce highly targeted immune responses, avoiding allergenic and/or reactogenic sequences. In this study, antigenic peptide sequence of Brucella abortus, WLAEIKQRSLMVHG, was chemically synthesized by adding tryptophan to the N-terminus of sequence, purified and characterized for the first time in the literature. Molecular weight of the peptide was determined by using LC–MS. A linear response (R2 > 0.998) is observed for peptide in the range of 2–250 µg/mL. The LOD and LOQ values are 0.08 and 0.27 µg/kg respectively. Precision and accuracy ranges were found to be %RSD < 0.2 and 57.3–103.2%, respectively. Fluorescence property of the peptide was shown via fluorescence spectroscopy measurement. The 3D de novo structure of the peptide was predicted with PEP-FOLD server. According to the data obtained from the lipophilicity study (LogD7.4 = − 3.093 ± 0.195), the peptide can not cross the blood–brain barrier if applied via intravenously. This has shown us that peptide can be used as a peptide candidate at the vaccine studies. The production of antigenic peptides as in this study is the main component of the preparation of synthetic vaccine systems.

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Acknowledgements

This work was supported by Research Fund of the Yildiz Technical University (Project Number: 2014-07-04-YL01) and the TUBITAK MSc Scholarship Program in Priority Areas (2210/C).

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Authors and Affiliations

  1. Bioengineering Department, Chemistry and Metallurgy Faculty, Yildiz Technical University, Istanbul, Turkey

    Tayfun Acar, Pelin Pelit Arayıcı, Burcu Ucar & Zeynep Mustafaeva

  2. Department of Nuclear Medicine and Molecular Imaging, VKF American Hospital, Istanbul, Turkey

    Burcu Ucar

  3. Department of Bioengineering, Faculty of Engineering and Natural Sciences, Uskudar University, Istanbul, Turkey

    Mesut Karahan

Authors
  1. Tayfun Acar
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  2. Pelin Pelit Arayıcı
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  3. Burcu Ucar
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  5. Zeynep Mustafaeva
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Correspondence to Tayfun Acar.

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Acar, T., Pelit Arayıcı, P., Ucar, B. et al. Synthesis, Characterization and Lipophilicity Study of Brucella abortus’ Immunogenic Peptide Sequence That Can Be Used in the Future Vaccination Studies. Int J Pept Res Ther 25, 911–918 (2019). https://doi.org/10.1007/s10989-018-9739-0

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  • DOI: https://doi.org/10.1007/s10989-018-9739-0

Keywords

  • Solid phase peptide synthesis (SPPS)
  • Brucella abortus
  • Peptide vaccine
  • LC–ESI–MS
  • Validation
  • Fluorescence
  • PEP-FOLD
  • Lipophilicity