Exogenous Catalase Stimulates the Polymyxin E-Induced Rapid Killing of Paenibacillus polymyxa
- 102 Downloads
Polymyxin E (colistin) has been considered as an important peptide antibiotic for clinical treatment of Gram-negative pathogens by selectively targeting lipopolysaccharide on the outer membrane. Recent reports showed that colistin can also kill Gram-positive bacteria, but the mechanism of colistin against Gram-positive bacteria remains unclear. In this study, we found that colistin can kill its producer Paenibacillus polymyxa, a Gram-positive bacterium, by both disrupting its cell integrity and inducing oxidative stress. Enhancement of the cell permeability is the major action of colistin against P. polymyxa. In relative, colistin-induced oxidative stress plays an associated role in killing of P. polymyxa. Notably, we, for the first time, found that exogenous catalase is able to intensify the colistin-induced killing by inhibiting the intracellular catalase activity and thus increasing oxidative stress. Our findings would shed light on a not yet clearly described bactericidal mechanism of colistin against Gram-positive bacteria. More importantly, our findings pave the road for the future to lower clinical use of colistin for avoiding bacterial resistance.
KeywordsColistin Catalase Membrane permeability Oxidative stress Paenibacillus polymyxa
This work was supported by National Natural Science Foundation of China (31670114) and Zhejiang Provincial Natural Science Foundation of China (LY16C010002) to ZY.
Compliance with Ethical Standards
Conflict of interest
The authors declare that they have no conflict of interest.
Research Involving Human and Animal Participants
This article does not contain any studies with human participants performed by any of the authors. We did not involve any animals as well human for this study.
- Repine JE, Fox RB, Berger EM (1981) Hydrogen peroxide kills Staphylococcus aureus by reacting with staphylococcal iron to form hydroxyl radical. J Biol Chem 256(14):7094–7096Google Scholar
- Rice LB (2006) Challenges in identifying new antimicrobial agents effective for treating infections with Acinetobacter baumannii and Pseudomonas aeruginosa. Clin Infect Dis 43(2):S100–S105Google Scholar
- Yu Z, Guo C, Qiu J (2015b) Precursor amino acids inhibit polymyxin E biosynthesis in Paenibacillus polymyxa, probably by affecting the expression of polymyxin E biosynthesis-associated genes. Biomed Res Int 2015:690830Google Scholar
- Yu Z, Qin W, Lin J, Fang S, Qiu J (2015c) Antibacterial mechanisms of polymyxin and bacterial resistance. Biomed Res Int 2015:679109Google Scholar
- Yu Z, Zhu Y, Qin W, Yin J, Qiu J (2017) Oxidative stress induced by polymyxin E involves in rapid killing of Paenibacillus polymyxa. Biomed Res Int 2017:5437139Google Scholar