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Bj-PRO-5a and Bj-PRO 10c Found at C-Type Natriuretic Peptide Precursor of Bothrops jararaca Change Renal Function of Hypertensive Rats

International Journal of Peptide Research and Therapeutics volume 23, pages 381–385 (2017)Cite this article

Abstract

Proline-rich oligopeptides from Bothrops jararaca (Bj-PROs) produce potent and long-lasting antihypertensive effect through mechanisms that go beyond ACE inhibition. In this study we evaluated the renal function parameters of spontaneously hypertensive rats (SHR) injected with Bj-PRO-5a and -10c (0.47, 71 or 710 nmol/kg) found in the CNP-precursor of the snake. At 71 and 710 nmol/kg, Bj-PROs increased urinary flow rate (18.1–43.5%). At 71 nmol/kg, Bj-PRO 5a and 10c elevated sodium excretion (68.1 and 40.9%, respectively) and Bj-PRO-5a also increased urinary sodium/creatinine ratio (56.5%). At 0.47 nmol/kg, Bj-PROs did not change renal function. All doses of Bj-PROs reduced blood pressure (Δ = −13 to −24mmHg). We conclude that Bj-PROs reduce blood pressure and improve renal function of SHRs through diuretic and natriuretic mechanisms.

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Fig. 1

Abbreviations

ACE:

Angiotensin I converting enzyme

AS:

Argininosuccinate synthatase

Bj :

Bothrops jararaca

CNP:

C-type natriuretic peptide

Cr:

Creatinine

GFR:

Glomerular filtration rate

Na:

Sodium

NO:

Nitric oxide

PRO:

Proline rich oligopeptide

SBP:

Systolic blood pressure

SHR:

Spontaneously hypertensive rat

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Acknowledgements

This work was supported by the Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP—CAT/Cepid, proc 98/14307-9 and Post-doctoral fellow proc. 08/54820-0); Fundação de Amparo à Pesquisa do Estado de Goiás (FAPEG—DCR 14/2013 and DOCFix 04/2014); Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and Coordenação de Pessoal de Nivel Superior (CAPES—Edital Toxinologia 63/2010—AUXPE 1593/2011).

Author Contributions

Participated in research design: DI, CHX, DAC-Jr and ACMC; Conducted experiments: JRRM, JY, KDD, CHX and DI; Contributed with new reagents or analytic tools: MMT, ACS, RASS and ACMC; Performed data analysis: CHX and DI; Wrote or contributed to the writing of the manuscript: CHX and DI.

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Authors and Affiliations

  1. Laboratory of Cardiovascular Physiology and Therapeutics, Department of Physiological Sciences, Institute of Biological Sciences, Federal University of Goias, sala 224. Av. Esperança, s/n, Goiania, GO, 74690-900, Brazil

    Carlos H. Xavier & Danielle Ianzer

  2. Special Laboratory of Applied Toxinology – CAT/Cepid-Fapesp, Butantan Institute, Sao Paulo, SP, 05503-900, Brazil

    José Rodolfo R. Miranda, Juliana Yamaguchi, Antônio C. M. Camargo & Danielle Ianzer

  3. Laboratory of Immunopharmacology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, Brazil

    Kátia D. da Silveira & Mauro M. Teixeira

  4. Department of Pediatrics, Faculty of Medicine, Federal University of Minas Gerais, Belo Horizonte, Brazil

    Ana C. Simões e Silva

  5. Laboratory of Hypertension, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, Brazil

    Robson A. S. Santos

  6. Departmento of Biological Sciences, Universidade Federal de Ouro Preto, Ouro Preto, Brazil

    Deoclécio Alves Chianca-Jr

Authors
  1. Carlos H. Xavier
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  2. José Rodolfo R. Miranda
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  3. Juliana Yamaguchi
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  4. Kátia D. da Silveira
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  5. Mauro M. Teixeira
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  6. Deoclécio Alves Chianca-Jr
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  7. Ana C. Simões e Silva
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  8. Robson A. S. Santos
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  9. Antônio C. M. Camargo
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  10. Danielle Ianzer
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Correspondence to Danielle Ianzer.

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Xavier, C.H., Miranda, J.R.R., Yamaguchi, J. et al. Bj-PRO-5a and Bj-PRO 10c Found at C-Type Natriuretic Peptide Precursor of Bothrops jararaca Change Renal Function of Hypertensive Rats. Int J Pept Res Ther 23, 381–385 (2017). https://doi.org/10.1007/s10989-017-9575-7

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Keywords

  • Bj-PROs
  • Blood pressure
  • Bradykinin-potentiating peptides
  • Heart rate
  • Hypertension
  • Proline-rich oligopeptides
  • Renal function