Abstract
This work, for the first time reports the optimization of rhG-CSF in Escherichia coli using novel media supplements. The rhG-CSF production was optimized using Plackett–Burman (P–B) and Central composite design (CCD) operated in an integrated process. Three novel media supplements (sodium pyruvate, methylglucoside and choline) along with two conventional essential elements (sodium citrate and magnesium sulphate) were screened with a P–B design. An integrated application of CCD represented a statistically significant response (R 2 = 98.56 % and P < 0.0001) for the factors under the study, that suggested a quadratic model for the prediction of rhG-CSF. The CCD results reveal the highest rhG-CSF production of 31.88 µg/mL with optimized components comprising 1.75 g/L of sodium pyruvate, 1.5 g/L of methylglucoside, 2.215 g/L of sodium citrate, 1.7 g/L of choline and 0.036 g/L of magnesium sulphate.
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References
Alam MZ, Jamal P, Nadzir MM (2008) Bioconversion of palm oil mill effluent for citric acid production: statistical optimization of fermentation media and time by central composite design. World J Microbiol Biotechnol 24:1177–1185
Amid A, Ismail NA, Yusof F, Salleh HM (2011) Expression, purification, and characterization of a recombinant stem bromelain from Ananas comosus. Process Biochem 46:2232–2239
Bari MN, Alam MZ, Muyibi SA, Jamal P (2009) Improvement of production of citric acid from oil palm empty fruit bunches: optimization of media by statistical experimental designs. Bioresour Technol 100:3113–3120
Bertho J-M, Frick J, Prat M, Demarquay C, Dudoignon N, Trompier F, Gorin NC, Thierry D, Gourmelon P (2005) Comparison of autologous cell therapy and granulocyte-colony stimulating factor (G-CSF) injection vs G-CSF injection alone for the treatment of acute radiation syndrome in a non-human primate model. Int J Radiat Oncol Biol Phys 63:911–920
Box GE, Wilson K (1951) On the experimental attainment of optimum conditions. J R Stat Soc Ser B 13:1–45
Caldas T, Demont-Caulet N, Ghazi A, Richarme G (1999) Thermoprotection by glycine betaine and choline. Microbiology 145:2543–2548
Carrió M, Villaverde A (2001) Protein aggregation as bacterial inclusion bodies is reversible. FEBS Lett 489:29–33
Cornell JA, Khuri AI (1987) Response surfaces: designs and analyses. Marcel Dekker, Inc., New York
Dagert M, Ehrlich S (1979) Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells. Gene 6:23–28
Deibel R, Niven C (1964) Pyruvate fermentation by Streptococcus faecalis. J Bacteriol 88:4–10
Fink AL (1998) Protein aggregation: folding aggregates, inclusion bodies and amyloid. Fold Des 3:R9–R23
Ghorbani F, Younesi H, Ghasempouri SM, Zinatizadeh AA, Amini M, Daneshi A (2008) Application of response surface methodology for optimization of cadmium biosorption in an aqueous solution by Saccharomyces cerevisiae. Chem Eng J 145:267–275
Karthikeyan S, Balasubramanian V, Rajendran R (2014) Developing empirical relationships to estimate porosity and microhardness of plasma-sprayed YSZ coatings. Ceram Int 40:3171–3183
Kennedy M, Krouse D (1999) Strategies for improving fermentation medium performance: a review. J Ind Microbiol Biotechnol 23:456–475
Kim CK, Lee CH, Lee S-B, Oh J-W (2013) Simplified large-scale refolding, purification, and characterization of recombinant human granulocyte-colony stimulating factor in Escherichia coli. PLoS One 8:e80109
Kobayashi SD, DeLeo FR (2009) Role of neutrophils in innate immunity: a systems biology-level approach. Wiley Interdiscip Rev 1:309–333
Kreth J, Lengeler JW, Jahreis K (2013) Characterization of pyruvate uptake in Escherichia coli K-12. PLoS One 8:e67125
Kurowski W, Fensom A, Pirt S (1975) Factors influencing the formation and stability of D-glucoside 3-dehydrogenase activity in cultures of Agrobacterium tumefaciens. J Gen Microbiol 90:191–202
Matthew S (1995) Taxol: science and applications. CRC Press, London
Muntari B, Amid A, Mel M, Jami MS, Salleh HM (2012) Recombinant bromelain production in Escherichia coli: process optimization in shake flask culture by response surface methodology. AMB Express 2:1–9
Shay L, Hunt H, Wegner G (1987) High-productivity fermentation process for cultivating industrial microorganisms. J Ind Microbiol 2:79–85
Sung L, Dror Y (2006) Clinical applications of granulocyte-colony stimulating factor. Front Biosci 12:1988–2002
Tanyildizi MS, Özer D, Elibol M (2005) Optimization of α-amylase production by Bacillus sp. using response surface methodology. Process Biochem 40:2291–2296
Tian H, Liu C, Gao X, Yao W (2013) Optimization of auto-induction medium for G-CSF production by Escherichia coli using artificial neural networks coupled with genetic algorithm. World J Microbiol Biotechnol 29:505–513
Tran C, Sly L, Mitchell D (1998) Selection of a strain of Aspergillus for the production of citric acid from pineapple waste in solid-state fermentation. World J Microbiol Biotechnol 14:399–404
Wilson K et al (2014) Bacterial expression of human kynurenine 3-monooxygenase: solubility, activity, purification. Protein Expr Purif 95:96–103
Yamanè T, Shimizu S (1984) Fed-batch techniques in microbial processes. In: Fiechter A (ed) Advances in biochemical engineering/biotechnology. Springer, Berlin, pp 147–194
Acknowledgments
The authors are grateful to the Department of Biotechnology and management of K L E F University, for providing all the financial assistance and laboratory facilities during execution of the work.
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Sandeep Vemula, Akshay Dedaniya, Rahul Bandaru, Maheswara Reddy Mallu, Vijaya Saradhi Settaluri and Srinivasa Reddy Ronda declare that they have no conflict of interest.
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Vemula, S., Dedaniya, A., Bandaru, R. et al. Production of Recombinant Human Granulocyte-Colony Stimulating Factor in Escherichia coli Using Novel Media Supplements Through Integrated Statistical Optimization Designs. Int J Pept Res Ther 21, 299–312 (2015). https://doi.org/10.1007/s10989-015-9458-8
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DOI: https://doi.org/10.1007/s10989-015-9458-8