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Antithrombotic Peptides from Scolopendra subspinipes mutilans Hydrolysates

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Abstract

The centipedes are a type of traditional Chinese herb and can treat cardiovascular diseases. In this study, we aimed to isolate bioactive peptides from the enzymatic hydrolysates of centipedes. Three novel antithrombotic peptides were isolated from centipedes hydrolysates using a combination of ultrafiltration, Sephadex G-50 column, and RP-HPLC C8 column. The molecular mass of the purified peptides are 753, 515 and 985 Da measured by Electrospray Ionization Mass Spectrometry. The primary structures of these peptides were determined to be Phe-Ser-Ala-Pro-Ala-Val-Tyr (FSAPAVY), Ile-Arg-Asp-Leu (IRDL) and Asp-Leu-Asp-His-Tyr-Ser-Phe (DLDHYSF) using Edman degradation method. All isolated peptides potently prolonged the activated partial thromboplastin time. Moreover, IRDL and DLDHYSF can inhibit platelet aggregation, prolong bleeding time and clotting time. These results indicate that the enzymatic hydrolysate of Scolopendra subspinipes mutilans may contribute to the antithrombotic activity of the centipede for treatment of cardiovascular and cerebrovascular diseases.

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Acknowledgments

This study was supported by Chinese National Natural Science Foundation (30972822, 81273274, and 81273375), Jiangsu Provincial Qing Lan Project, The Innovative Plan of Jiangsu Provincial Graduate (No. CXZZ11-0822). All institutional and national guidelines for the care and use of laboratory animals were followed.

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All the authors declare that they have no conflict of interest

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Authors and Affiliations

  1. School of Life Science & Technology, China Pharmaceutical University, 24 Tong Jia Xiang, Nanjing, 210009, People’s Republic of China

    Yi Kong, Shuai Li, Yu Shao, Zhi-long He & Mei-mei Chen

  2. State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, 210009, People’s Republic of China

    Yi Kong

  3. Division of Molecular Pharmaceutics, UNC Eshelman School of Pharmacy, The University of North Carolina at Chapel Hill, Chapel Hill, NC, 27599, USA

    Xin Ming

  4. Research Division of Clinical Pharmacology, the First Affiliated Hospital with Nanjing Medical University, 300 Guangzhou Road, Nanjing, 210029, People’s Republic of China

    Ji-fu Wei

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  1. Yi Kong
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  2. Shuai Li
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  3. Yu Shao
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  4. Zhi-long He
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  5. Mei-mei Chen
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  6. Xin Ming
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  7. Ji-fu Wei
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Correspondence to Yi Kong or Ji-fu Wei.

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Kong, Y., Li, S., Shao, Y. et al. Antithrombotic Peptides from Scolopendra subspinipes mutilans Hydrolysates. Int J Pept Res Ther 20, 245–252 (2014). https://doi.org/10.1007/s10989-013-9387-3

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  • DOI: https://doi.org/10.1007/s10989-013-9387-3

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