Abstract
An immunosensor with rapid and ultrasensitive response for vascular endothelial growth factor (VEGF) has been built up with 4-aminothiophenol (4-ATP) onto the gold surfaces. Quantitative analysis of VEGF was performed by recording the impedance changing of the gold electrode surface by binding of VEGF. The human vascular endothelial growth factor receptor 1 (VEGF-R1, Flt-1) was used as a biorecognition element for the first time in the literature. VEGF-R1 was covalently immobilized via 4-ATP self-assembled monolayer formed on gold thin film covered surface. Construction of the biosensor was carefully characterised by the techniques such as electrochemistry and electrochemical impedance spectroscopy. In order to characterize impedance data, Kramers–Kronig transform was performed on the experimental impedance data. The limit of detection of the immunosensor for qualitative detection was 100 pg/mL while the LOD for quantitative detection could down to 100 pg/mL by using the VEGF-R1 based biosensor. Finally, artificial serum samples spiked with VEGF was analyzed by the proposed immunosensor to investigate useful of the biosensor for early biomarker diagnosis.
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Acknowledgments
The authors greatly appreciate the support of the TÜBİTAK (The Scientific and Technological Research Council of Turkey, Project number: 109 T 172).
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Mustafa Teke, Çiğdem Sayıklı, Çetin Canbaz, Mustafa Kemal Sezgintürk declare that they have no conflict of interest.
Informed Consent
All procedures followed were in accordance with the ethical standards of the responsible committee on human experimentation (institutional and national) and with the Helsinki Declaration of 1975, as revised in 2000 (5). Informed consent was obtained from all patients for being included in the study.
Statement of Human and Animal Rights
All institutional and national guidelines for the care and use of laboratory animals were followed. All procedures followed were in accordance with the ethical standards of the responsible committee on human experimentation (institutional and national) and with the Helsinki Declaration of 1975, as revised in 2008 (5). Informed consent was obtained from all patients for being included in the study.
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Teke, M., Sayıklı, Ç., Canbaz, Ç. et al. A Novel Biosensing System Using Biological Receptor for Analysis of Vascular Endothelial Growth Factor. Int J Pept Res Ther 20, 221–230 (2014). https://doi.org/10.1007/s10989-013-9386-4
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DOI: https://doi.org/10.1007/s10989-013-9386-4