Structure and ACE-Inhibitory Activity of Peptides Derived from Hen Egg White Lysozyme

  • Mina Memarpoor-Yazdi
  • Ahmad Asoodeh
  • JamshidKhan Chamani

DOI: 10.1007/s10989-012-9311-2

Cite this article as:
Memarpoor-Yazdi, M., Asoodeh, A. & Chamani, J. Int J Pept Res Ther (2012) 18: 353. doi:10.1007/s10989-012-9311-2


Angiotensin I-converting enzyme plays an important role in hypertension and therefore its inhibition is considered to be a useful procedure in the prevention of hypertension. Two novel ACE inhibitory peptides were purified and identified from the papain-trypsin hydrolysate of hen egg white lysozyme using reverse phase-high performance liquid chromatography. The sequences of identified peptides were NTDGSTDYGILQINSR (MW: 1,753.98 ± 0.5 Da) and VFGR (MW: 459.26 ± 0.5 Da), which were named F2 and F9 peptide, respectively. Analyses of the far-UV CD spectra of ACE in the absence and presence of the F2 peptide revealed ACE secondary structural changes. In the presence of the F2 peptide, a loss of helical content of ACE was observed, which can lead to decrease of the enzymatic activity. Lineweaver–Burk plots show that the identified peptides both act as non-competitive ACE inhibitors. These findings would be helpful on the understanding of interaction between ACE and its inhibitory peptides.


RP-HPLC ACE inhibitory activity Inhibition pattern Circular dichroism spectroscopy 



Angiotensin I-converting enzyme




Reverse phase high-performance liquid chromatography


Hen egg white lysozyme


Circular dichroism


Matrix-assisted laser desorption/ionization time of flight

Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Mina Memarpoor-Yazdi
    • 1
  • Ahmad Asoodeh
    • 2
    • 3
  • JamshidKhan Chamani
    • 1
  1. 1.Department of Biology, Faculty of Sciences, Mashhad BranchIslamic Azad UniversityMashhadIran
  2. 2.Department of Chemistry, Faculty of SciencesFerdowsi University of MashhadMashhadIran
  3. 3.Cellular and Molecular Research Group, Institute of BiotechnologyFerdowsi University of MashhadMashhadIran

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