The Chemical Synthesis of the Collagenous Domain of the Hormone Adiponectin
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As a first step towards the preparation of a functional biomolecule, a chemical synthesis of the collagenous domain of adiponectin is described. The 76 residue polypeptide (without post-translational modifications) could be assembled efficiently from smaller unprotected peptides using two native chemical ligation reactions followed by a reductive desulfurisation step. In turn, the polypeptides were synthesised in high purity by microwave enhanced solid phase synthesis.
KeywordsAdiponectin Native chemical ligation Collagenous
The authors wish to thank The Maurice Wilkins Centre for Molecular Biodiscovery for financial support.