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The Chemical Synthesis of the Collagenous Domain of the Hormone Adiponectin

  • Paul W. R. Harris
  • Margaret A. Brimble
Article

Abstract

As a first step towards the preparation of a functional biomolecule, a chemical synthesis of the collagenous domain of adiponectin is described. The 76 residue polypeptide (without post-translational modifications) could be assembled efficiently from smaller unprotected peptides using two native chemical ligation reactions followed by a reductive desulfurisation step. In turn, the polypeptides were synthesised in high purity by microwave enhanced solid phase synthesis.

Keywords

Adiponectin Native chemical ligation Collagenous 

Notes

Acknowledgments

The authors wish to thank The Maurice Wilkins Centre for Molecular Biodiscovery for financial support.

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  1. 1.School of Chemical Sciences and Maurice Wilkins Centre for Molecular BiodiscoveryThe University of AucklandAucklandNew Zealand

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