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Hydrolysis of DNA by a Branched Peptide without Aromatic Residues

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International Journal of Peptide Research and Therapeutics Aims and scope Submit manuscript

A branched peptide Nα, Nɛ-di (l-leucyl)-l-lysine was found to efficiently cleave supercoiled double-strand DNA such as PUC19 DNA at optimum pH 4.0 in 40 mmol/l Britton–Robbinson buffer. The T4 ligase experiment implied that the DNA cleavage occurs via a hydrolytic path. The dependence of the cleavage reaction on the ionic strength indicated that the interaction of DNA with the branched peptide involve only electrostatic binding.

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Authors and Affiliations

  1. The Key Laboratory of Bioorganic Phosphorus, Chemical Biology, Ministry of Education, Tsinghua University, Beijing, 100084, China

    Anshan Xiao, Yuping Feng, Wenjun Xie & Yufen Zhao

  2. Department of Chemistry, Capital Normal University, 100037, Beijing, China

    Shengli Cao

Authors
  1. Anshan Xiao
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  2. Yuping Feng
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  3. Shengli Cao
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  4. Wenjun Xie
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  5. Yufen Zhao
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Correspondence to Yuping Feng.

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Xiao, A., Feng, Y., Cao, S. et al. Hydrolysis of DNA by a Branched Peptide without Aromatic Residues. Int J Pept Res Ther 11, 181–183 (2005). https://doi.org/10.1007/s10989-005-6788-y

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  • DOI: https://doi.org/10.1007/s10989-005-6788-y

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