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Surface plasmon resonance analysis of antimicrobial peptide–membrane interactions: affinity & mechanism of action

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Abstract

Antimicrobial peptides are being increasingly recognised as potential candidates for antibacterial drugs in the face of the rapidly emerging bacterial resistance to conventional antibiotics in recent years. However, a precise understanding of the relationship between antimicrobial peptide structure and their cytolytic function in a range of organisms is still lacking. This is a result of the complex nature of the interactions of antimicrobial peptides with the cell membrane, the mechanism of which can vary considerably between different classes of antimicrobial peptides. A wide range of biophysical techniques have been used to study the influence of a number of peptide and membrane properties on the cytolytic activity of these peptides model membrane systems. Until recently, however, very few studies had reported measurements of the affinity of antimicrobial peptides for different membrane systems mainly due to the difficulty in obtaining this information. Surface plasmon resonance (SPR) spectroscopy has recently been applied to the study of biomembrane-based systems which has allowed a real-time analysis of binding affinity and kinetics. This mini review provides an overview of the recent applications that demonstrate the potential of SPR to study the membrane interactions of antimicrobial peptides.

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Authors and Affiliations

  1. Department of Biochemistry and Molecular Biology, Monash University, Clayton, Victoria, 3800, Australia

    Kristopher Hall, Henriette Mozsolits & Marie-Isabel Aguilar

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  1. Kristopher Hall
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  2. Henriette Mozsolits
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  3. Marie-Isabel Aguilar
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Hall, K., Mozsolits, H. & Aguilar, MI. Surface plasmon resonance analysis of antimicrobial peptide–membrane interactions: affinity & mechanism of action. Int J Pept Res Ther 10, 475–485 (2003). https://doi.org/10.1007/s10989-004-2407-6

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  • DOI: https://doi.org/10.1007/s10989-004-2407-6

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