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Repeated bout effect on the cytoskeletal proteins titin, desmin, and dystrophin in rat skeletal muscle

Abstract

The aim of this study was to evaluate the effect of repeated bouts of exercise on the cytoskeletal proteins titin, desmin, and dystrophin. Rats were made to run downhill for 90 min 1 or 5 times separated by 14 days. Samples were taken from quadriceps femoris muscle 3, 48, 96 h and 50 days after the last exercise session and detected by quantitative PCR, histochemical stainings, and western blot analyses. Histopathological changes in titin, desmin, and dystophin stainings, an increase in β-glucuronidase activity (a quantitative indicator of muscle damage), a significant decrease in the relative content of dystrophin, and intramyocellular Evans blue staining (signs of changes in sarcolemmal permeability) observed after one exercise session were attenuated after 5 exercise sessions. Titin mRNA level was not increased after the initial exercise session but was increased after the fifth session. Desmin and dystrophin mRNA levels were increased after the first and fifth sessions with desmin showing a smaller increase after the fifth session compared to the first session. Prior exercise induces adaptation that protects the sarcolemma as well as subsarcolemmal, intermediate filament, and sarcomeric proteins against disruption. Changes in mRNA levels of titin, desmin, and dystophin after an acute exercise session obviously reflect the need of these proteins in the repair process following damage. After five sessions increase in mRNA of studied proteins suggest a strong involvement in continuing adaptation to the increased exercise.

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Acknowledgements

This study was supported by the LIKES Foundation and the Ministry of Education, Finland.

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Correspondence to T. Maarit Lehti.

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Lehti, T.M., Kalliokoski, R. & Komulainen, J. Repeated bout effect on the cytoskeletal proteins titin, desmin, and dystrophin in rat skeletal muscle. J Muscle Res Cell Motil 28, 39–47 (2007). https://doi.org/10.1007/s10974-007-9102-0

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  • DOI: https://doi.org/10.1007/s10974-007-9102-0

Keywords

  • Adaptation
  • Downhill running
  • Gene expression
  • Histochemistry