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Characteristics of muscle fibers reconstituted in the regeneration process of masseter muscle in an mdx mouse model of muscular dystrophy

Journal of Muscle Research & Cell Motility volume 27, pages 235–240 (2006)Cite this article

Abstract

Mdx mice, which lack dystrophin, were examined for changes in the properties of muscle fibers in the growth process of the masseter muscle at the morphological, protein and transcriptional levels. The slow-type isoform, MyHC-1, and the fast-type isoforms, MyHC-2a, MyHC-2d and MyHC-2b, were examined at the protein and the transcriptional level. Morphological examination showed that in the mdx mouse masseter muscle, degeneration, necrosis, and regeneration occurred, particularly at the age of 4 weeks, and many regenerated muscle fibers with centrally located nuclei were observed at the age of 9 weeks. The results of examination at the protein and the transcriptional level showed that in the process of muscle fiber degeneration, necrosis, and regeneration, the mdx mouse masseter muscle acquires muscle fiber characteristics entirely different from those in the normal mouse masseter muscle. In particular, MyHC-1, which is rarely found in normal mice, was very strongly expressed.

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References

  • Abe S, Maejima M, Watanabe H, Shibahara T, Agematsu H, Doi T, Sakiyama K, Usami A, Gojo K, Hashimoto M, Yoshinari M, Ide Y (2002) Muscle-fiber characteristics in the adult mouse tongue muscles. Anat Sci Int 77:145–148

    PubMed  Article  Google Scholar 

  • Bottinelli R, Schiaffino S, Reggiani C (1991) Force–velocity relations and myosin heavy chain isoform compositions of skinned fibers from rat skeletal muscle. J Physiol 437:655–672

    PubMed  CAS  Google Scholar 

  • Brueckner JK, Itkis O, Porter PD (1996) Spatial and temporal patterns of myosin heavy chain expression in developing rat extraocular muscle. J Muscle Res Cell Motil 17:297–312

    PubMed  CAS  Article  Google Scholar 

  • Bulfield G, Siller WG, Wight PA, Moore KJ (1984) X chromosome-linked muscular dystrophy (mdx) in the mouse. Proc Natl Acad Sci USA 81:1189–1192

    PubMed  CAS  Article  Google Scholar 

  • Dangain J, Vrbova G (1984) Muscle development in mdx mutant mice. Muscle Nerve 7:700–704

    PubMed  CAS  Article  Google Scholar 

  • DiMario JX, Uzman A, Strohman RC (1991) Fiber regeneration is not persistent in dystrophic (mdx) mouse skeletal muscle. Dev Biol 148:314–321

    PubMed  CAS  Article  Google Scholar 

  • Doi T, Abe S, Ide Y (2003) Masticatory function and properties of masseter muscle fibers in microphthalmia (mi/mi) mice during postnatal development. Ann Anat 185:435–440

    PubMed  Article  Google Scholar 

  • Eason JM, Schwartz GA, Pavlath GK, English AW (2000) Sexually dimorphic expression of myosin heavy chains in the adult mouse masseter. J Appl Physiol 89:251–258

    PubMed  CAS  Google Scholar 

  • Elbrink J, Malhotra SK (1985) The pathogenesis of Duchenne muscular dystrophy: significance of experimental observations. Med Hypotheses 17:375–385

    PubMed  CAS  Article  Google Scholar 

  • Gojo S, Abe S, Ide Y (2002) Characteristics of myofibers in the masseter muscle of mice during postnatal growth. Anat Histol Embryol 31:1–9

    Article  Google Scholar 

  • Hori A, Ishihara A, Kobayashi S, Ibata Y (1998) Immunohistochemical classification of skeletal muscle fibers. Acta Histochem Cytochem 31:375–384

    CAS  Google Scholar 

  • Maejima M, Abe S, Sakiyama K, Agematsu H, Hashimoto M, Tamatsu Y, Ide Y (2005) Changes in tongue muscle fiber properties of mouse before and after weaning. Arch Oral Biol 50:988–993

    PubMed  CAS  Article  Google Scholar 

  • Marques MJ, Lus MA, Minatel E, Neto HS (2005) Muscle regeneration in dystrophic mdx mice is enhanced by isosorbide dinitrate. Neurosci Lett 382:342–345

    PubMed  CAS  Article  Google Scholar 

  • Matsuda S, Desaki J, Fujita H, Okumura N, Sakanaka M (1992) Immuno-electron-microscopic localization of basic fibroblast growth factor in the dystrophic mdx mouse masseter muscle. Cell Tissue Res 270:569–576

    PubMed  CAS  Article  Google Scholar 

  • Muller J, Vayssiere N, Royuela M, Leger ME, Muller A, Bacou F, Pons F, Hugon G, Mornet D (2001) Comparative evolution of muscular dystrophy in diaphragm, gastrocnemius and masseter muscles from old male mdx mice. J Muscle Res Cell Motil 22:133–139

    PubMed  CAS  Article  Google Scholar 

  • Pastoret C, Sebille A (1995) Mdx mice show progressive weakness and muscle deterioration with age. J Neurol Sci 129:97–105

    PubMed  CAS  Article  Google Scholar 

  • Pette D, Sarton RS (1990) Cellular and molecular diversities of mammalian skeletal muscle fibers. Rev Physiol Biochem Pharmacol 116:1–76

    PubMed  CAS  Google Scholar 

  • Sakiyama K, Abe S, Tamatsu Y, Ide Y (2005) Effects of stretching stress on the muscle contraction proteins of skeletal muscle myoblasts. Biomed Res 26:61–68

    PubMed  CAS  Article  Google Scholar 

  • Sartorius CA, Lu B, Acakpo-Satchivi L, Jacobsen RP, Byrnes C, Leinwand LA (1998) Myosin heavy chain 2a and 2d are functionally distinct in mouse. J Cell Biol 141:943–953

    PubMed  CAS  Article  Google Scholar 

  • Schiaffino S, Gorza L, Sartore S, Saggin L, Ausoni S, Vianello M, Gundersen K, Lomo T (1989) Three myosin heavy chain isoforms in type 2 skeletal muscle fibers. J Muscle Res Cell Motil 10:197–205

    PubMed  CAS  Article  Google Scholar 

  • Schiaffino S, Reggiani C (1996) Molecular diversity of myofibrillar proteins: gene regulation and functional significance. Physiol Rev 76:371–425

    PubMed  CAS  Google Scholar 

  • Shida T, Abe S, Sakiyama K, Agematsu H, Mitarashi S, Tamatsu Y, Ide Y (2005) Superficial and deep layer muscle-fiber properties of the mouse masseter before and after weaning. Arch Oral Biol 50:65–71

    PubMed  CAS  Article  Google Scholar 

  • Tanabe Y, Esaki K, Nomura T (1986) Skeletal muscle pathology in X chromosome-linked muscular dystrophy (mdx) mouse. Acta Neuropathol 69:91–95

    PubMed  CAS  Article  Google Scholar 

  • Talmandge RJ, Roy RR (1993) Electrophoretic separation of rat skeletal muscle myosin heavy-chain isoforms. J Appl Physiol 75:2337–2340

    Google Scholar 

  • Tuxen A, Kirkeby S (1990) An animal model for human masseter muscle: histochemical characterization of mouse, rat, rabbit, cat, dog, pig, and cow masseter muscle. J Oral Maxillofac Surg 48:1063–1067

    PubMed  CAS  Article  Google Scholar 

  • Usami A, Abe S, Ide Y (2003) Myosin heavy chain isoforms of the murine masseter muscle during pre- and post-natal development. Anat Histol Embryol 32:244–248

    PubMed  CAS  Article  Google Scholar 

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Acknowledgements

This study was supported by grants-in-aid for scientific research (14704046: S.A.) from the Ministry of Education, Culture, Sports, Science and Technology, Japan.

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Authors and Affiliations

  1. Department of Anatomy, Tokyo Dental College, 1-2-2, Masago, Mihama-ku, Chiba, 261-8502, Japan

    Won-Hyung Lee, Shinichi Abe, Hee-Jin Kim, Akinobu Usami, Atsuro Honda, Koji Sakiyama & Yoshinobu Ide

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  1. Won-Hyung Lee
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  2. Shinichi Abe
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  3. Hee-Jin Kim
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  4. Akinobu Usami
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  6. Koji Sakiyama
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  7. Yoshinobu Ide
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Correspondence to Shinichi Abe.

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Lee, WH., Abe, S., Kim, HJ. et al. Characteristics of muscle fibers reconstituted in the regeneration process of masseter muscle in an mdx mouse model of muscular dystrophy. J Muscle Res Cell Motil 27, 235–240 (2006). https://doi.org/10.1007/s10974-006-9066-5

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  • DOI: https://doi.org/10.1007/s10974-006-9066-5

Keywords

  • Myosin heavy chain
  • MyHC2a
  • MyHC2b
  • Mdx
  • LightCycler®