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Does phosphate release limit the ATPases of soleus myofibrils? Evidence that (A)M· ADP·Pi states predominate on the cross-bridge cycle

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Abstract

The ATPases (±Ca2+) of myofibrils from rabbit soleus (a slow muscle) and psoas (a fast muscle) have different E a: −Ca2+, 78 and 60 kJ/mol and +Ca2+, 155 and 71 kJ/mol, respectively. At physiological temperatures, the two types of myofibrillar ATPase are very similar and yet the mechanical properties of the muscles are different (Candau et al. (2003) Biophys J 85: 3132–3141). Muscle contraction relies on specific interactions of the different chemical states on the myosin head ATPase pathway with the thin filament. An explanation for the E a data is that different states populate the pathways of the two types of myofibril because the rate limiting steps are different. Here, we put this to the test by a comparison of the transient kinetics of the initial steps of the ATPases of the two types of myofibril at 4°C. We used two methods: rapid flow quench (`cold ATP chase': titration of active sites, ATP binding kinetics, k cat; `Pi burst': ATP cleavage kinetics) and fluorescence stopped-flow (MDCC-phosphate binding protein for free Pi; myofibrillar tryptophan fluorescence for myosin head-thin filament detachment and ATP cleavage kinetics). We find that, as with psoas myofibrils, the most populated state on the cross-bridge cycle of soleus myofibrils, whether relaxed or activated, is (A)M·ADP·Pi. We propose a reaction pathway that includes several (A)M·ADP·Pi sub-states that are either `weak' or `strong', depending on the mechanical condition.

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Authors and Affiliations

  1. UMR, 5121, France

    Bogdan Iorga, Franck Travers, Tom Barman & Corinne Lionne

  2. CNRS-Université Montpellier I, Institut de Biologie, 4 boulevard Henri IV (CS89508), France

    Robin Candau

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  1. Bogdan Iorga
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  2. Robin Candau
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  3. Franck Travers
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  4. Tom Barman
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  5. Corinne Lionne
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Iorga, B., Candau, R., Travers, F. et al. Does phosphate release limit the ATPases of soleus myofibrils? Evidence that (A)M· ADP·Pi states predominate on the cross-bridge cycle. J Muscle Res Cell Motil 25, 367–378 (2004). https://doi.org/10.1007/s10974-004-0812-2

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