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DSC analysis of the influence of time and concentration of Stryphnodendron adstringens extract on corneal cross-linking

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Abstract

Keratoconus is an ectasia that leads to reduced stiffness of the cornea, which acquires a conical shape. Many studies are being carried out aiming at new treatments that lead to the recovery of these cross-links. Therefore, the efficiency of the Stryphnodendron adstringens butanolic extract in promoting cross-linking in corneal stroma of porcine eyes was analyzed. The influence of concentration of this plant extract to form cross-links was analyzed, as well as the influence of the contact time. The denaturation temperature results obtained for corneas treated with 1, 2, or 4% of the barbatimão butanolic extract show an increase of 3.84, 11.37, and 33.23%, respectively. These results suggest a rise in the number of cross-links. As for the contact time of the cornea with the 2% solution of the butanolic extract, it appears that the ideal time was 60 min. Enzymatic digestion assays confirm a decrease of 39% and 59% in the relative area and relative mass of corneas in the control group, while for the group treated with the barbatimão extract, these values were close to 0% and 9%, respectively. Therefore, it was possible to verify the efficiency of the barbatimão butanolic extract in modifying the stroma of porcine corneas, which promoted an increase in resistance to thermal and enzymatic degradation.

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Acknowledgements

This work was supported by the grant #2016/19933-5 from São Paulo Research Foundation (FAPESP).

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Authors and Affiliations

  1. Departamento de Bioquímica, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, Brazil

    Patrícia Alessandra Bersanetti

  2. Departamento de Oftalmologia e Ciências Visuais, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, Brazil

    Aline de Assis Marquini

  3. Departamento de Engenharia Química, Centro Universitário FEI, São Bernardo do Campo, Brazil

    Andreia de Araújo Morandim-Giannetti

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  1. Patrícia Alessandra Bersanetti
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All authors contributed to the study conception and design. Material preparation, data collection, and analyses were performed by PAB, AAM, and AAM-G. The first draft of the manuscript was written by AAM-G and PAB, and all authors commented on previous versions of the manuscript. All authors have read and approved the final manuscript.

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Correspondence to Patrícia Alessandra Bersanetti.

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Bersanetti, P.A., Marquini, A.d. & Morandim-Giannetti, A.d. DSC analysis of the influence of time and concentration of Stryphnodendron adstringens extract on corneal cross-linking. J Therm Anal Calorim 148, 191–195 (2023). https://doi.org/10.1007/s10973-022-11730-9

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