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Ionic liquid synergistic effect between preparation of hybrid supports and immobilization of lipase applied to esters production

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Abstract

The present work aims to evaluate the synergistic effect of imidazolium-based ionic liquids (IL) in the preparation and immobilization of Burkholderia cepacia lipase in a new hybrid support of poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) and silica. In addition, these biocatalysts were applied in transesterification reactions of crude coconut oil for the production of ethyl esters. Lipase immobilized on hybrid support with [C4min][NTf2] or [C6min][NTf2] ionic liquid exhibited nearly ≈ 1.6-fold immobilization yield greater than IB-Control. The use of IL during the preparation of the hybrid support promoted changes in its morphological structure as presented by B.E.T. and FTIR. Thermogravimetric analyzes were used to characterize the prepared derivatives and support the results of immobilization yields and the synergic effect of IL used. Transesterification reactions applying biocatalysts showed that the conversion maximum in esters (59%) was found under conditions of molar ratio 1:7 (oil:ethanol) in 72 h of reaction. These results were obtained using [C4min][NTf2] during the preparation of the hybrid support for the immobilization of Burkholderia cepacia lipase, thus showing the potential of these new biocatalysts.

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Acknowledgements

This study ws financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior—Brasil (CAPES)—Finance Code 001; and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq).

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Authors and Affiliations

  1. Universidade Tiradentes, Av. Murilo Dantas 300, Farolândia, 49032-490, SE, Brasil

    Sílvia R. S. Martins, Suellen M. S. Andrade, Alini T. Fricks, Álvaro S. Lima, Renan T. Figueiredo, Cleide M. F. Soares & Ranyere L. Souza

  2. Instituto de Tecnologia E Pesquisa, Av. Murilo Dantas 300 - Prédio do ITP - Farolândia, 49032-490, Aracaju, SE, Brasil

    Alini T. Fricks, Álvaro S. Lima, Renan T. Figueiredo, Cleide M. F. Soares & Ranyere L. Souza

  3. Centro de Ciências Exatas E Tecnologia, Universidade Federal de Sergipe, Av. Marechal Rondon s/n, Jardim Rosa Elze, 49100-000, São Cristóvão, SE, Brasil

    Daniel P. Silva & Lisiane S. Freitas

  4. Instituto de Química, Universidade Federal de Mato Grosso Do Sul, Avenida Senador Filinto Müller 1555, Cidade Universitária, Campo Grande, 79074-460, MS, Brasil

    Rebeca Y. Cabrera-Padilla

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Martins, S.R.S., Andrade, S.M.S., Fricks, A.T. et al. Ionic liquid synergistic effect between preparation of hybrid supports and immobilization of lipase applied to esters production. J Therm Anal Calorim 147, 1143–1156 (2022). https://doi.org/10.1007/s10973-020-10408-4

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