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Attainment and characterization of carboxymethyl chitosan hydrogels by enzymatic cross-linking

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Abstract

In this study, carboxymethyl chitosan hydrogels (CMCH) cross-linked with catechin in the presence of laccase (CMCH-C-L) were obtained. The synthesis and characterization of CMCH were carried out from chitosan (CH) and its cross-linking with catechin (C) in the presence of laccase (L). DSC, ATR-FTIR, and rheological analyses of the CMCH solution and CMCH-C-L hydrogel were carried out. Three main thermal events were observed in the DSC characterization with temperatures and enthalpies related to each event for CMCH: 167.2 °C/+ 523.3 J g−1, 269.1 °C/− 35.0 J g−1, and 333.3 °C/− 17.6 J g−1, possibly referring to these processes: polymerization, breaking of electrostatic interactions, and decomposition of the material, respectively. The CMCH-C-L hydrogel presented values of 165.2 °C/+ 376.6 J g−1, 266.7 °C/− 47.8 J g−1, and 333.3 °C/− 31.5 J g−1. Conversely, the chitosan presented only one thermal event of decomposition at 302.5 °C and enthalpy of − 258.5 J g−1. Rheological properties of the hydrogels (viscosities, consistency values, and flow indices) showed non-Newtonian pseudoplastic rheological behavior and that the viscosity increased approximately 150% after the reaction (134.25 mPa s for CMCH and 342.85 mPa s in CMCH-C-L) confirming the formation of cross-links after the addition of catechin and laccase. Morphological analyses of CMCH and CMCH-C-L films via SEM showed the modification of the material after the reaction with the catechin in the presence of laccase and corroborated with the results, confirming the presence of cross-links.

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Acknowledgements

This work was supported by the Grant # 2015/19273-2 from the São Paulo Research Foundation (FAPESP). We thank Dr. Nivaldo Boralle and Silvia Helena Santagneli for the NMR measurements and LMA-IQ UNESP-Araraquara-SP, Brazil, for making the high-resolution electronic scanning microscope available.

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Authors and Affiliations

  1. Department of Chemical Engineering, Centro Universitário FEI, São Bernardo do Campo, São Paulo, Brazil

    Andreia de Araújo Morandim-Giannetti & Patrick de Oliveira Wecchi

  2. Department of Health Informatics, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, Brazil

    Paula de Araújo Silvério

  3. Department of Ophthalmology and Visual Sciences, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, Brazil

    Regina Carlstron

  4. Department of Biochemistry, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, Brazil

    Patrícia Alessandra Bersanetti

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  1. Andreia de Araújo Morandim-Giannetti
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  2. Patrick de Oliveira Wecchi
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  3. Paula de Araújo Silvério
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Correspondence to Andreia de Araújo Morandim-Giannetti.

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Morandim-Giannetti, A.d., Wecchi, P.d., Silvério, P.d. et al. Attainment and characterization of carboxymethyl chitosan hydrogels by enzymatic cross-linking. J Therm Anal Calorim 138, 3635–3643 (2019). https://doi.org/10.1007/s10973-019-08571-4

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  • DOI: https://doi.org/10.1007/s10973-019-08571-4

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