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Microcalorimetric study on the activation effects of Salviae miltiorrhizae combined with Radix puerariae on mice splenic lymphocytes

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Abstract

The thermogenic activity of Radix puerariae, Salvia miltiorrhiza and S. miltiorrhiza + R. puerariae on the spleen lymphocytes of mice was investigated by using isothermal microcalorimetry. By analyzing the area under the curve, a good dose–effect relationship was obtained for R. puerariae, S. miltiorrhiza and S. miltiorrhiza + R. puerariae, respectively. R. puerariae group and S. miltiorrhiza group expressed a synergistic effect on the spleen lymphocytes between R. puerariae and S. miltiorrhiza. The effect is in a high-dose-dependent relationship. Group of the S. miltiorrhiza + R. puerariae combination was significantly better than the effect of low-dose group, in line with the clinical drug rule. The activation mechanism was explored by network pharmacology method. The 20 pathways which S. miltiorrhiza and R. puerariae work together may be the basis for their synergistic effects. The combined application of microcalorimetry and network pharmacology of traditional Chinese medicine provides new ideas and research directions for traditional Chinese medicine researches.

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Acknowledgements

This study was supported by Foundation of National Major Drug Discovery Project (No. 2015ZX09J15102-004), Science and technology project of Liaoning Province (NO. 201602275).

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Authors and Affiliations

  1. College of Pharmacy, Jinzhou Medical University, Jinzhou, China

    Xiangbo Meng & Chunmei Dai

  2. Department of Pharmacy, 302 Military Hospital of China, Beijing, China

    Xiangbo Meng, Xuelin Zhou, Tao Wang, Haotian Li, Shizhang Wei, Huandan Cai & Yanling Zhao

  3. Treatment and Research Center for Infectious Diseases, 302 Hospital of P.L.A., Beijing, China

    Fengyi Li

  4. Integrative Medical Center, 302 Military Hospital of China, Beijing, China

    Jianyu Li & Ruilin Wang

  5. China Military Institute of Chinese Medicine, 302 Military Hospital of China, Beijing, China

    Yaming Zhang

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  1. Xiangbo Meng
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Correspondence to Chunmei Dai or Yanling Zhao.

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Meng, X., Zhou, X., Wang, T. et al. Microcalorimetric study on the activation effects of Salviae miltiorrhizae combined with Radix puerariae on mice splenic lymphocytes. J Therm Anal Calorim 137, 841–848 (2019). https://doi.org/10.1007/s10973-018-7949-5

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  • DOI: https://doi.org/10.1007/s10973-018-7949-5

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