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Microcalorimetric method to determine ceftazidime and piperacillin–tazobactam susceptibility in Pseudomonas aeruginosa

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Abstract

Pseudomonas aeruginosa is a gram-negative bacterium, considered one of the most common causes of nosocomial infections. Bacterial cultures produce an exchange of energy as a result of their metabolism. The rate of heat production is an adequate measure of the metabolic activity of the organisms and their constituent parts. Micro-organisms produce small amounts of heat, in the order of 1–3 pW per cell. Although the heat produced by bacteria is very small, their exponential reproduction in a culture medium permits heat detection through microcalorimetry. In this work, we analysed the microcalorimetric behaviour of P. aeruginosa and its susceptibility against two antibiotics: ceftazidime and piperacillin–tazobactam. We performed this experiment five times using different concentrations of antibiotics (2 × MIC, 1 × MIC, 0.75 × MIC, 0.5 × MIC y 0.25 × MIC). The effect of antibiotics on bacterial growth can be evaluated by the delay of heat flow and reduction of the heat flow peak compared to results with the growth control without the presence of antibiotics. The measures were carried out using a Calvet microcalorimeter that contains two stainless steel cells of approximately 10 cm3 (reference and experimental). A constant temperature of 309.65 K was maintained within the microcalorimeter. Recording the difference in calorific potential over time, we obtained the growth curves of P. aeruginosa.

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Acknowledgements

We thank María Perfecta Salgado Gonzalez and Sofia Baz Rodríguez for their collaboration with the technical measures. We are also thankful for the financial support provided by the projects EM 2012/141, CN 2012/285, and “Agrupación Estratégica de Biomedicina (INBIOMED)” by “Xunta de Galicia” and the project FIS 2011-23322 funded by Ministry of Science and Innovation of Spain. All these projects are co-financed with FEDER funds.

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Authors and Affiliations

  1. Pharmacy Service, Vigo University Hospital, Pizarro nº 22, 36204, Vigo, Spain

    L. Esarte López & N. Lago Rivero

  2. Department of Applied Physics, University of Vigo, Lagoas Marcosende, 36310, Vigo, Spain

    C. Vázquez, M. M. Mato Corzón & J. L. Legido Soto

Authors
  1. L. Esarte López
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  2. N. Lago Rivero
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  3. C. Vázquez
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  4. M. M. Mato Corzón
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  5. J. L. Legido Soto
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Correspondence to C. Vázquez.

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Esarte López, L., Lago Rivero, N., Vázquez, C. et al. Microcalorimetric method to determine ceftazidime and piperacillin–tazobactam susceptibility in Pseudomonas aeruginosa . J Therm Anal Calorim 121, 353–360 (2015). https://doi.org/10.1007/s10973-015-4685-y

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  • DOI: https://doi.org/10.1007/s10973-015-4685-y

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