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Influence of oxidative injury and monitoring of blood plasma by DSC on patients with psoriasis

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Abstract

Oxidative stress induced by oxygen free radicals (OFRs) is a casual factor in psoriasis. Our aim was to detect the oxidative stress parameters and blood plasma changes with differential scanning calorimetry (DSC) in psoriatic patients. The study included untreated (n = 39) and treated (retinoids, methotrexate, biologic response modifiers; n = 33) white adult patients from both sex. To monitor oxidative stress concentration of malondialdehyde (MDA), reduced glutathione and sulfhydryl groups, production of OFRs, and activity of myeloperoxidase (MPO), superoxide dismutase, and catalase were measured. Denaturation of plasma components was detected in SETARAM Micro DSC-II calorimeter. Total production of OFRs and MPO activity, and the concentration of MDA were significantly increased both in untreated patients with moderate and severe symptoms and in all drug-treated groups compared with controls (p < 0.001). All of the scavengers and antioxidants were significantly decreased in untreated patients and better preserved after retinoid and biological therapy. DSC scans of blood plasma showed melting temperature a characteristic parameter to follow the severity of disease. The calorimetric enthalpy is exhibiting a moderate decrease with the progression of the inflammation. These findings suggest that an imbalance exists between pro-oxidants and antioxidants in untreated severe psoriatic patients. All drug therapy reduced the changes, mainly the biologic response modifiers. Similarly, DSC showed differences between untreated and conventional systemic drug treatment.

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Acknowledgements

This work was supported by Grants OTKA CO-272 (for Dénes Lőrinczy). The present scientific contribution is dedicated to the 650th anniversary of the foundation of the University of Pécs, Hungary.

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Authors and Affiliations

  1. Department of Dermatology, Venereology and Oncodermatology, Medical School, University of Pécs, Akác Str. 1, Pecs, 7632, Hungary

    Medhi Moezzi

  2. Surgery Clinic, Medical School, University of Pécs, Rákóczi Str. 2, Pecs, 7624, Hungary

    István Zapf

  3. Government Office of Baranya County, József A. Str. 10, Pecs, 7623, Hungary

    Tamás Fekecs

  4. General and Vascular Surgical Department, Zala County Hospital, Zrínyi Str. 1, Zalaegerszeg, 8900, Hungary

    Klára Nedvig

  5. Department of Biophysics, Medical School, University of Pécs, Szigeti Str. 12, Pecs, 7624, Hungary

    Dénes Lőrinczy

  6. Department of Surgical Research and Techniques, Medical Faculty, Semmelweis University, Nagyvárad Sqr. 4, Budapest, 1089, Hungary

    Andrea Ferencz

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  1. Medhi Moezzi
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  2. István Zapf
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  5. Dénes Lőrinczy
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  6. Andrea Ferencz
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Correspondence to Andrea Ferencz.

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Moezzi, M., Zapf, I., Fekecs, T. et al. Influence of oxidative injury and monitoring of blood plasma by DSC on patients with psoriasis. J Therm Anal Calorim 123, 2037–2043 (2016). https://doi.org/10.1007/s10973-015-4674-1

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  • DOI: https://doi.org/10.1007/s10973-015-4674-1

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