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Isothermal microcalorimetric investigation of the toxic action of the effective constituents of Podophyllum emodi Wall on Tetrahymena thermophila BF5

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Abstract

The effects of podophyllotoxin (PPT), etoposide (VP16), and teniposide (VM26) on the growth of Tetrahymena thermophila BF5 (T.t.BF5) was investigated by the TAM AIR isothermal microcalorimetric system. The extent and duration of toxic effects on T.t.BF5 metabolism were evaluated by studying the growth rate constant (k), inhibitory ratio (I), maximum heat-output power (P max), peak time of maximum heat-output power (t p), and total heat production (Q t). Experimental results showed that the value of t p increased and the value of k and P max decreased with the increasing compound concentrations. Furthermore, the growth rate constant k was linear with compound concentration. The corresponding I was obtained from different k values. According to the IC10 (the concentration of inhibitor when the inhibitory ratio is 10 %), the relative toxicity of the three compounds was PPT (IC10 = 49.6 μg mL−1) > VP16 (IC10 = 117.5 μg mL−1) > VM26 (IC10 = 359.1 μg mL−1). The preliminary investigation of structure–activity relationships showed that the thienyl group was likely responsible for reducing the toxicity of the compounds.

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Acknowledgements

The authors gratefully acknowledge the financial support of the National Science and Technology Major Project of China (2012ZX10005-005).

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Authors and Affiliations

  1. Integrative Medicine Specialty, Chinese PLA Medical School, Beijing, 100853, People’s Republic of China

    M. Gong

  2. Integrative Medical Center for Liver Diseases, 302 Hospital of PLA, Beijing, 100039, People’s Republic of China

    M. Gong, N. Zhang, Y.-Q. Sun, S.-Q. Luo, Y.-L. Zhao, D. Yan, X.-H. Xiao & J. Li

  3. National Center of Biomedical Analysis, Beijing, 100850, People’s Republic of China

    X. Z. Wang

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  1. M. Gong
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  2. N. Zhang
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  5. Y.-L. Zhao
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Correspondence to D. Yan.

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Gong, M., Zhang, N., Sun, YQ. et al. Isothermal microcalorimetric investigation of the toxic action of the effective constituents of Podophyllum emodi Wall on Tetrahymena thermophila BF5 . J Therm Anal Calorim 115, 2369–2374 (2014). https://doi.org/10.1007/s10973-013-3314-x

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  • DOI: https://doi.org/10.1007/s10973-013-3314-x

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