Skip to main content
SpringerLink
Log in

Ethanolic extracts of Moringa oleifera Lam.

Evaluation of its potential as an antioxidant additive for fish oil

  • Published:
Journal of Thermal Analysis and Calorimetry Aims and scope Submit manuscript

Abstract

This paper reports the evaluation of the antioxidant potential of the ethanolic extracts of the leaves (EL), flowers (EF), seed pods (EP) and seeds (ES) from Moringa oleifera Lam. The antioxidant potential was assessed, upon the addition of the extracts to fish oil, by means of the total extractable phenol content (TEP), the DPPH· free radical scavenging efficiency and using pressurized differential scanning calorimetry (P-DSC). The results of TEP and DPPH· showed that the ES extract does not present a potential to be used as an antioxidant additive, and it was thus discarded from the remaining analyses. Thus, the following treatments were prepared: pure fish oil, fish oil with BHT (100 mg kg−1), fish oil with TBHQ (100 mg kg−1) and fish oil with EL, EF and EP—all at the concentration of 100 mg kg−1, in relation to the total extractable phenolics contained in each one of the extracts. The leaf and flower extracts displayed a protecting effect, with an increase in about 20 and 11 % of the OIT values, respectively. However, such protection was smaller than that conferred by the synthetic antioxidants utilized. As for the thermal analysis results, it was noticed that EL presented the highest thermal stability among the extracts.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  1. Lukiw WJ, Bazan NG. Docosahexaenoic acid and the aging brain. Am Soc Nutr. 2008;138:2510–4.

    CAS  Google Scholar 

  2. Nemets H, Nemets B, Apter A, Bracha Z, Belmaker RH. Omega-3 treatment of childhood depression: a controlled, double-blind pilot study. Am J Psychiatr. 2006;163:1098–100.

    Article  Google Scholar 

  3. Morris MC, Evans D, Tangney CC, Bienias JL, Wilson RS. Fish consumption and cognitive decline with age in a large community study. Arch Neurol. 2005;62:1849–53.

    Article  Google Scholar 

  4. Bykerk VP, Keystone EC. What are the goals and principles of management in the early treatment of rheumatoid arthritis? Best practice and research. Clinic Rheumat. 2005;19:147–61.

    Google Scholar 

  5. Kris-etherton PM, Hecker KD, Bonanome A, Coval SM, Binkoski AE, Hilpert KF, Griel AE, Etherton TD. Bioactive compounds in foods : their role in the prevention of cardiovascular disease and cancer. Am J Med. 2002;113:71–88.

    Article  Google Scholar 

  6. Pivik RT, Dykman RA, Jing H, Gilchrist JM, Badger TM. Early infant diet and the omega 3 fatty acid DHA: effects on resting cardiovascular activity and behavioral development during the first half-year of life. Dev Neuropsychol. 2009;34:139–58.

    Article  CAS  Google Scholar 

  7. Balk EM, Lichtenstein AH, Chung M, Kupelnick B, Chew P, Lau J. Effects of omega-3 fatty acids on serum markers of cardiovascular disease risk: a systematic review. Atherosclerosis. 2006;189:19–30.

    Article  CAS  Google Scholar 

  8. Mothé CG, Castro BCS, Mothé MG. Characterization by TG/DTG/DSC and FTIR of frying and fish oil residues to obtain biodiesel. J Therm Anal Calorim. 2011;106:811–7.

    Article  Google Scholar 

  9. Barriuso B, Astiasarán I, Ansorena D. A review of analytical methods measuring lipid oxidation status in foods: a challenging task. Eur Food Res Technol. 2013;236:1–15.

    Article  CAS  Google Scholar 

  10. Siriwardhana N, Lee KW, Kim SH, Há JH, Park GT, Jeon YJ. Lipid peroxidation inhibitory effects of Hizikia fusiformis methanolic extract on fish oil and linoleic acid. Food Sci Technol Int. 2004;10:65–72.

    Article  CAS  Google Scholar 

  11. Hras AR, Hadolin M, Knez Z, Bauman D. Comparison of antioxidative and synergistic effects of rosemary extract with a-tocopherol, ascorbyl palmitate and citric acid in sunflower oil. Food Chem. 2000;71:229–33.

    Article  CAS  Google Scholar 

  12. Brasil. Ministério da Saúde. Comissão Nacional de Normas e Padrões para Alimentos. Resolução no. 04/88. In: Associação Brasileira das Indústrias de Alimentação, Compêndio da Legislação de Alimentos. São Paulo: ABIA, 2001, v. 1, p. 3.26.

  13. Brewer MS. Natural Antioxidants: sources, Compounds, Mechanisms of Action, and Potential Applications. Compr Rev Food Sci F. 2011;10:221–47.

    Article  CAS  Google Scholar 

  14. Iqbal S, Bhanger MI. Effect of season and production location on antioxidant activity of Moringa oleifera leaves grown in Pakistan. J Food Compos Anal. 2006;19:544–51.

    Article  CAS  Google Scholar 

  15. Lako J, Trenerry V, Wahlqvist M, Wattanapenpaiboon N, Sotheeswaran S, Premier R. Phytochemical flavonols, carotenoids and the antioxidant properties of a wide selection of Fijian fruit, vegetables and other readily available foods. Food Chem. 2007;101:1727–41.

    Article  CAS  Google Scholar 

  16. Reddy V, Urooj A, Kumar A. Evaluation of antioxidant activity of some plant extracts and their application in biscuits. Food Chem. 2005;90:317–21.

    Article  CAS  Google Scholar 

  17. Slinkard K, Singleton VL. Total phenol analysis: automation and comparison with manual methods. Am J Enol Vitic. 1977;28:49–55.

    CAS  Google Scholar 

  18. Blois MS. Antioxidant determinations by the use of a stable free radical. Nature. 1958;181:1199–200.

    Article  CAS  Google Scholar 

  19. Fresco P, Borges F, Diniz C, Marques MPM. New insights on the anticancer properties of dietary polyphenols. Medic Res Rev. 2006;26:747–66.

    Article  CAS  Google Scholar 

  20. Singh BN, Singh BR, Singh RL, Prakash D, Dhakarey R, Upadhyay G, Singh HB. Oxidative DNA damage protective activity, antioxidant and anti-quorum sensing potentials of Moringa oleifera. Food Chem Toxicol. 2009;47:1109–16.

    Article  CAS  Google Scholar 

  21. Angelo PM, Jorge N. Evaluation of stored sunflower oil with the addition of antioxidants. Ciênc Tecnol Aliment. 2008;28:498–502.

    Article  CAS  Google Scholar 

  22. Contreras-Calderón J, Calderón-Jaimes L, Guerra-Hernández E, García-Villanova B. Antioxidant capacity, phenolic content and vitamin C in pulp, peel and seed from 24 exotic fruits from Colombia. Food Res Int. 2011;44:2047–53.

    Article  Google Scholar 

  23. Santos NA, Cordeiro AMTM, Damasceno SS, Aguiar RT, Rosenhaim R, Carvalho Filho JR, Santos IMG, Maia AS, Souza AG. Commercial antioxidants and thermal stability evaluations. Fuel. 2012;97:638–43.

    Article  CAS  Google Scholar 

  24. Fernandes FHA, Santana CP, Santos RL, Correia LP, Conceição MM, Macêdo RO, Medeiros ACD. Thermal characterization of dried extract of medicinal plant by DSC and analytical techniques. J Therm Anal Calorim. 2012;. doi:10.1007/s10973-012-2807-3.

    Google Scholar 

  25. Araújo KLGV, Epaminondas PS, Silva MCD, Lima AEA, Rosenhaim R, Maia AS, Soledade LEB, Souza AL, Santos IMG, Souza AG, Queiroz N. Influence of thermal degradation in the physicochemical properties of fish oil. J Therm Anal Calorim. 2011;106:557–61.

    Article  Google Scholar 

  26. Leonardo RS, Murta Valle ML, Dweck J. An alternative method by pressurized DSC to evaluate biodiesel antioxidants efficiency. J Therm Anal Calorim. 2012;108:751–9.

    Article  CAS  Google Scholar 

  27. Bennett RN, Mellon FA, Foidl N, Pratt JH, Dupont MS, Perkins L, Kroon PA. Profiling glucosinolates and phenolics in vegetative and reproductive tissues of the multi-purpose trees Moringa oleifera L. (horseradish tree) and Moringa stenopetala L. J Agric Food Chem. 2003;51:3546–53.

    Article  CAS  Google Scholar 

Download references

Acknowledgments

The authors acknowledge the Brazilian agencies Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) for the financial support.

Author information

Authors and Affiliations

  1. Programa de Pós-Graduação em Ciência e Tecnologia de Alimentos, CT, Campus I, Universidade Federal da Paraíba, João Pessoa, Paraíba, 58051-970, Brazil

    Jaqueline A. Nascimento, Kassandra L. G. V. Araújo, Poliana S. Epaminondas, Alline S. Souza & Marciane Magnani

  2. Laboratório de Combustíveis e Materiais—LACOM, Departamento de Química, CCEN, Campus I, Universidade Federal da Paraíba, João Pessoa, Paraíba, 58059-900, Brazil

    Antonia L. Souza, Neide Queiroz & Antonio G. Souza

  3. Setor de Agroindústria, Instituto Federal de Educação, Ciência e Tecnologia (IFPB), Campus Sousa, Sousa, Paraíba, 58800-970, Brazil

    Poliana S. Epaminondas

  4. Campus de Pinheiro, Universidade Federal do Maranhão, Pinheiro, Maranhão, 65200–000, Brazil

    Luiz E. B. Soledade

Authors
  1. Jaqueline A. Nascimento
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Kassandra L. G. V. Araújo
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Poliana S. Epaminondas
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Alline S. Souza
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Marciane Magnani
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Antonia L. Souza
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Luiz E. B. Soledade
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Neide Queiroz
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Antonio G. Souza
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Jaqueline A. Nascimento.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Nascimento, J.A., Araújo, K.L.G.V., Epaminondas, P.S. et al. Ethanolic extracts of Moringa oleifera Lam.. J Therm Anal Calorim 114, 833–838 (2013). https://doi.org/10.1007/s10973-013-3045-z

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10973-013-3045-z

Keywords

Search

Navigation