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DSC and Raman study on the effect of lysozyme and bovine serum albumin on phospholipids liposomes

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Abstract

In this paper, the effect of increasing amounts of lysozyme (Lyso) and bovine serum albumin (BSA) on the behaviour of lecithin (DMPC) and cephalin (DMPE) liposomes was investigated by means of Raman and DSC techniques. The results showed that both proteins affected, but in a different way, both lecithin and cephalin liposomes. In the samples with lower Lyso concentrations (up to 2 % w/w), a small decrease on the main transition temperature (T m) was observed, whereas T m increased by further addition of Lyso (up to 15.0 % w/w). At the same time, an increase of about 20 % in the ΔH of the transition was observed. Pre-transition was also affected in a greater extent by protein presence. When BSA interacted with liposomes, a smaller increase in the T m values was observed with a contemporary increase of about 8 % in the associated ΔH. The data suggested that the BSA–liposomes interaction involves only the external surface of the bilayer, excluding thus any penetration into the liposomal hydrophobic core. On the contrary, a partial penetration into the bilayer is suggested when Lyso is added to liposomes. Both considered proteins strengthened the overall bilayer structure of DMPC liposomes, suggesting a decrease in the membrane permeability. Moreover, Lyso secondary structure changed by interaction with liposomes, as demonstrated by the Raman spectra behaviour, in particular in the case of DMPE.

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Acknowledgements

This work was supported by grants from Bologna University (Ricerca fondamentale orientata—ex 60 %).

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  1. Department of Biochemistry ‘G. Moruzzi’, University of Bologna, Via Belmeloro 8/2, 40126, Bologna, Italy

    M. Di Foggia, S. Bonora & V. Tugnoli

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  1. M. Di Foggia
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  2. S. Bonora
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  3. V. Tugnoli
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Correspondence to M. Di Foggia.

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Di Foggia, M., Bonora, S. & Tugnoli, V. DSC and Raman study on the effect of lysozyme and bovine serum albumin on phospholipids liposomes. J Therm Anal Calorim 111, 1871–1880 (2013). https://doi.org/10.1007/s10973-012-2842-0

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  • DOI: https://doi.org/10.1007/s10973-012-2842-0

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