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Thermal behavior and signature patterns of human cytokine and soluble cytokine receptors investigated using dielectric thermal analysis and thermogravimetry

Journal of Thermal Analysis and Calorimetry volume 108, pages 41–51 (2012)Cite this article

Abstract

Cytokines are small regulatory proteins secreted mostly by cells of the immune system. Cytokines participate in anti-inflammatory and pro-inflammatory processes in the body and in responses to host exposure to pathogens. In this study, the thermal behavior of human recombinant cytokines and soluble cytokine receptors; IFNγ, TNFα, IL-1 receptor antagonist, soluble TNF-receptor types 1 and 2, and sIL-2 receptor α were analyzed by dielectric thermal analysis at 37 °C and by thermogravimetry. Measurements were performed at a frequency range of 0.1–300,000 Hz. Permittivity and loss factor measurements were used to calculate mobility of charges (tan δ values) in the proteins from Debye plots. Peak frequencies and polarization times were used to determine dielectric signatures for each cytokine and receptor. Peak frequencies and polarization times were obtained for each cytokine and receptor analyzed. Detection of unique dielectric signatures of the proteins will aid development of sensitive dielectric sensors capable of detecting cytokines and soluble cytokine receptors in various human samples for malaria diagnosis.

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Acknowledgements

Funding for this research was provided by Cleveland State University’s 2010 Undergraduate Summer Research Program. We thank Ellen Matthews and Naullage I. Perera for helpful discussions.

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Authors and Affiliations

  1. Department of Biological, Geological, and Environmental Sciences, College of Science and Health Professions, Cleveland State University, Cleveland, OH, 44115, USA

    Salaam W. Saleh, Susan E. Moreno-Molek & Tobili Sam-Yellowe

  2. Department of Chemistry, College of Science and Health Professions, Cleveland State University, Cleveland, OH, 44115, USA

    Druthiman Reddy Mantheni, Manik Pavan Kumar Maheswaram & Alan T. Riga

Authors
  1. Salaam W. Saleh
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  2. Susan E. Moreno-Molek
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  3. Druthiman Reddy Mantheni
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  4. Manik Pavan Kumar Maheswaram
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  5. Tobili Sam-Yellowe
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  6. Alan T. Riga
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Correspondence to Tobili Sam-Yellowe or Alan T. Riga.

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Saleh, S.W., Moreno-Molek, S.E., Mantheni, D.R. et al. Thermal behavior and signature patterns of human cytokine and soluble cytokine receptors investigated using dielectric thermal analysis and thermogravimetry. J Therm Anal Calorim 108, 41–51 (2012). https://doi.org/10.1007/s10973-011-1530-9

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  • DOI: https://doi.org/10.1007/s10973-011-1530-9

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