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Inter-monomer cross-linking affects the thermal transitions in F-actin

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Abstract

Chemical cross-links which covalently connected the Cys-374 and Glu-41 residues of adjacent monomers in the same strand of F-actin were used to follow the consequences of the modification for the motional and structural properties of the actin filaments. DSC measurements reported that the inter-monomer cross-links shifted the thermal transition temperature and affected strongly the cooperativity of the transition in comparison with uncross-linked F-actin. Addition of HMM to F-actin induced significant decrease of the transition temperature to lower value from 69.4 to 67. 5 °C.

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Acknowledgements

The SETARAM Micro DSC-II used in the experiments were purchased with fund provided by the National Research Foundation Grant CO-272.

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Authors and Affiliations

  1. Institute of Forensic Medicine, Faculty of Medicine, University of Pecs, Szigeti u. 12, Pecs, 7633, Hungary

    F. Könczöl

  2. Biophysical Department, Faculty of Medicine, Institute of Biophysics, University of Pecs, Szigeti u. 12, Pecs, 7633, Hungary

    D. Lőrinczy & J. Belagyi

  3. Institute of Physiology, Faculty of Medicine, University of Pecs, Szigeti u. 12, Pecs, 7633, Hungary

    Zs. Vértes

  4. Department of Biochemistry, Eötvös University, Pázmány Péter sétány 1, Budapest, 1117, Hungary

    G. Hegyi

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  1. F. Könczöl
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  2. D. Lőrinczy
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  3. Zs. Vértes
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  4. G. Hegyi
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  5. J. Belagyi
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Correspondence to D. Lőrinczy.

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Könczöl, F., Lőrinczy, D., Vértes, Z. et al. Inter-monomer cross-linking affects the thermal transitions in F-actin. J Therm Anal Calorim 101, 549–553 (2010). https://doi.org/10.1007/s10973-010-0833-6

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  • DOI: https://doi.org/10.1007/s10973-010-0833-6

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