Journal of Thermal Analysis and Calorimetry

, Volume 102, Issue 1, pp 49–55 | Cite as

Anti-fungal effect of berberine on Candida albicans by microcalorimetry with correspondence analysis

  • Yanling Zhao
  • Dan Yan
  • Jiabo Wang
  • Ping Zhang
  • Xiaohe Xiao
Article

Abstract

Using a LKB-2277 bioactivity monitor, stop-flow mode, the power–time curves of Candida albicans growth at 37 °C affected by berberine were measured. The check experiments were studied based on agar cup method to observe the inhibitory diameter and serial dilution method to determine the minimal inhibitory concentration (MIC) of berberine on C. albicans growth. By analyzing the quantitative thermogenic parameters taken from the power–time curves using correspondence analysis (CA), we could find that berberine at a low concentration (5.0 μg mL−1) began to inhibit the growth of C. albicans and at a high concentration (75.0 μg mL−1) completely inhibited C. albicans growth. The anti-fungal activity of berberine could also be expressed as half-inhibitory concentration IC50, i.e., 50% effective in this inhibition. The value of IC50 of berberine on C. albicans was 34.52 μg mL−1. The inhibitory diameters all exceeded 10 mm in test range and the MIC was 500 μg mL−1. Berberine had strong anti-fungal effect on C. albicans growth. This work provided an important idea of the combination of microcalorimetry and CA for the study on anti-fungal effect of berberine and other compounds. Compared with the agar cup method and serial dilution method, microcalorimetry not only offered a useful way for evaluating the bioactivity of drugs, but also provides more information about the microbial growth and all this information was significant for the synthesis and searching of antibiotics.

Keywords

Berberine Anti-fungal activity Microcalorimetry Correspondence analysis 

References

  1. 1.
    Yang Y, Ye XL, Li XG, Zhen LS. Anti-microbial effect of four alkaloids from Coptidis rhizoma. Med Mater Med Res. 2007;18:3013–4.Google Scholar
  2. 2.
    Nishida S, Kikuichi S, Yoshioka S, Tsubaki M, Fujii Y, Matsuda H, et al. Induction of apoptosis in HL-60 cells treated with medicinal herbs. Am J Chin Med. 2003;31:551–62.CrossRefGoogle Scholar
  3. 3.
    Yokozawa T, Ishida A, Kashiwada Y, Cho EJ, Kim HY, Ikeshiro Y. Coptidis rhizoma: protective effects against peroxynitrite-induced oxidative damage and elucidation of its active components. J Pharm Pharmacol. 2004;56:547–56.CrossRefGoogle Scholar
  4. 4.
    Ye BN, Hao ML, Liu P, Xie F. Action mechanism of anti-inflammation effect of berberine. Chin J Veter Med. 2008;44:85–6.Google Scholar
  5. 5.
    Weber HA, Zart MK, Hodges AE, Molloy HM, Brien BM, Moody LA, et al. Chemical comparison of goldenseal (Hydrastis canadensis L.) root powder from three commercial suppliers. J Agric Food Chem. 2003;51:7352–8.CrossRefGoogle Scholar
  6. 6.
    Hwang JM, Kuo HC, Tseng TH, Liu JY, Chu CY. Berberine induces apoptosis through a mitochondria/caspases pathway in human hepatoma cells. Arch Toxicol. 2006;80:62–73.CrossRefGoogle Scholar
  7. 7.
    Hsu WH, Hsieh YS, Kuo HC, Teng CY, Huang HI, Wang CJ, et al. Berberine induces apoptosis in SW620 human colonic carcinoma cells through generation of reactive oxygen species and activation of JNK/p38 MAPK and FasL. Arch Toxicol. 2007;81:719–28.CrossRefGoogle Scholar
  8. 8.
    Zhang XD, Ren HM, Liu L, Qu CX. Effect of berberine on heart failure. China Pharma. 2007;16:19–20.Google Scholar
  9. 9.
    Tai WP, Tiang G, Huang YB, Zhou J, Zhang TC, Luo HS. The inhibitory effect of berberine on cyclooxygenase-2/Ca2+ pathway in human colon cell line. Chin Pharm Bull. 2005;21:950–3.Google Scholar
  10. 10.
    Wu J, Wang KX, Li CP, Wang J, Zhu YX, Hu YY. Anti-bacterial activity of Coptis chinensis and berberine on Helicobacter pylori in vitro. Pharm Clin Chin Mater Med. 2006;22:37–8.Google Scholar
  11. 11.
    Batovska D, Parushev St, Slavova A, Bankova V, Tsvetkova I, Ninova M, et al. Study on the substituents’ effects of a series of synthetic chalcones against the yeast Candida albicans. Eur J Med Chem. 2007;42:87–92.CrossRefGoogle Scholar
  12. 12.
    Zhou WC, Zhou HY. Survey of syntheses of azole antifungals. Chin J Pharm. 2006;37:125–33.Google Scholar
  13. 13.
    Leng P, Guo XL, Yang Y, Lou HX. Primary study on anti-fungal activities and reversal of fluconazole resistance of Plagiochin E. Chin Pharm J. 2007;42:349–52.Google Scholar
  14. 14.
    Crocco EI, Mimica LMJ, Muramati LH, Garcia C, Souza VM, Ruiz LRB, et al. Identification of Candida species and antifungal susceptibility in vitro: a study on 100 patients with superficial candidiasis. An Bras Dermatol. 2004;79:689–97.CrossRefGoogle Scholar
  15. 15.
    Yang LN, Xu F, Sun LX, Zhao ZB, Song CG. Microcalorimetric studies on the antimicrobial actions of different cephalosporins. J Therm Anal Calorim. 2008;93:417–21.CrossRefGoogle Scholar
  16. 16.
    Yao J, Liu Y, Liang HG, Zhang C, Zhu JZ, Qin X, et al. The effect of Zinc(II) on the growth of E. coli studied by microcalorimetry. J Therm Anal Calorim. 2005;79:39–43.CrossRefGoogle Scholar
  17. 17.
    Kong WJ, Zhao YL, Shan LM, Xiao XH, Guo WY. Thermochemical studies on the quantity-antibacterial effect relationship of four organic acids from Radix isatidis on Escherichia coli growth. Biol Pharm Bull. 2008;31:1301–5.CrossRefGoogle Scholar
  18. 18.
    Ruan L, Wang Y, Wai L, Wai L, Fu YH. Microcalorimetric research on recombinant Escherichia coli with high production of polyhydroxyalkanoates (PHAs). J Therm Anal Calorim. 2007;89:953–6.CrossRefGoogle Scholar
  19. 19.
    Chen XJ, Feng WS, Miao W, Yu YH, Shen YF, Wan CY, et al. A microcalorimetric assay of Tetrahymena thermophila for assessing tributyltin acute toxicity. J Therm Anal Calorim. 2008;94:779–84.CrossRefGoogle Scholar
  20. 20.
    Antonelli ML, Tornelli RF. Calorimetric analysis of three hydroxy acids as markers for quality and safety in food. J Therm Anal Calorim. 2008;91:113–7.CrossRefGoogle Scholar
  21. 21.
    Liu Q, Li L, Chen ZL. Research of the anti-candida Aibicans effect of Rhizoma coptidis in vitro. China Pharm. 2004;13:26–7.Google Scholar
  22. 22.
    Kong WJ, Zhao YL, Shan LM, Xiao XH, Guo WY. Investigation on the spectrum-effect relationships of EtOAc extract from Radix isatidis based on HPLC fingerprints and microcalorimetry. J Chromatogr B. 2008;871:109–14.CrossRefGoogle Scholar
  23. 23.
    Kong WJ, Zhao YL, Xiao XH, Wang JB, Li HB, Li ZL, et al. Spectrum-effect relationships between ultra performance liquid chromatography fingerprints and anti-bacterial activities of Rhizoma coptidis. Anal Chim Acta. 2009;634:279–85.CrossRefGoogle Scholar
  24. 24.
    Yan D, Han YM, Wei L, Xiao XH. Effect of berberine alkaloids on Bifidobacterium adolescentis growth by microcalorimetry. J Therm Anal Calorim. 2009;95:495–9.CrossRefGoogle Scholar
  25. 25.
    Xie CL, Tang HK, Song ZH, Qu SS. Microcalorimetric study of bacterial growth. Thermochim Acta. 1988;123:33–7.CrossRefGoogle Scholar
  26. 26.
    Spooner FD, Sykes G, Norris JR, Ribbons DW. Methods in microbiology, vol. 7B. London: Academic Press; 1972. p. 216.Google Scholar
  27. 27.
    Hindler J, Isenberg HD. Clinical microbiology procedures handbook. Washington, DC: American Society of Microbiology; 1992.Google Scholar
  28. 28.
    Luo S, Chen JW, Ma JL. Application of log-linear model and correspondence analysis on the clinical contingency table document. J Math Med. 2008;21:13–4.Google Scholar
  29. 29.
    Liu P, Wang FY. Correspondence analysis on trace elements of wolfberry fruit in different regions. J Anhui Agri Sci. 2008;36:8155–7.Google Scholar
  30. 30.
    Lu YJ, Li XZ, Wu QK. Action mechanism of berberine alkaloids. Chin J Nat Med. 1993;63:14–7.Google Scholar
  31. 31.
    Iwasa K, Kamigauchi M, Ueki M, Taniguchi M. Antibacterial activity and structure–activity relationships of berberine analogs. Eur J Med Chem. 1996;31:469–78.CrossRefGoogle Scholar
  32. 32.
    Donlan RM, Costerton JW. Biofilms: survival mechanisms of clinically relevant microorganisms. Clin Microbiol Rev. 2002;15:l67–93.CrossRefGoogle Scholar
  33. 33.
    Rokar R, Vivoda M, Kmetec V. Use of isothermal microcalorimetry for prediction of oxidative stability of several amino acids. J Therm Anal Calorim. 2008;92:791–4.CrossRefGoogle Scholar
  34. 34.
    Kong WJ, Zhao YL, Xiao XH, Li ZL, Ren YS. Action of palmatine on Tetrahymena thermophila BF5 growth investigated by microcalorimetry. J Hazard Mater. 2009;168:609–13.CrossRefGoogle Scholar

Copyright information

© Akadémiai Kiadó, Budapest, Hungary 2009

Authors and Affiliations

  • Yanling Zhao
    • 1
  • Dan Yan
    • 1
  • Jiabo Wang
    • 1
  • Ping Zhang
    • 1
  • Xiaohe Xiao
    • 1
  1. 1.China Military Institute of Chinese Materia MedicaBeijingPeople’s Republic of China

Personalised recommendations