Abstract
HPLC and UV spectrophotometry were developed and validated for quantitative determination of two antihiperlipoproteinemia drugs, lovastatin and simvastatin.
Analytical performance parameters such as linearity, precision, specificity, limit of detection (LOD) and limit of quantification (LOQ) were determined according to the ICH Q1B guidelines. Chromatography was carried out by isocratic technique on a reversed-phase C-18 column. The UV spectroscopy determinations were performed at 238 nm. The linearity of the calibration curves in the desired concentration range was good (r(2)>0.999) for both HPLC and UV methods. The relative standard deviation (RSD) for these methods was <5%. Moreover, the precision obtained with HPLC correlated well with the UV results. The methods proposed are highly sensitive and precise. Other methods used for assessment of the photostability of the substances studied were electron paramagnetic resonance (EPR) and differential scanning calorimetry (DSC).
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R. Paoletti, A. Corsini and S. Bellosta, Atherosclerosis Supplements, 3 (2002) 35.
A. S. Wierzbicki, R. Poston and A. Ferro, Pharmacology and Therapeutics, 99 (2003) 95.
G. Schmitz and T. Langmann, Vascular Pharmacology, 44 (2006) 75.
G. Beck, K. Kesseler, E. Baader, W. Bartmann, A. Bergmann, E. Granzer, H. Jendralla, B. Von Kerekjarto, R. Krause, G. Beck, K. Kesseler, E. Baader, W. Bartmann, A. Bergmann, E. Granzer, H. Jendralla, B. Von Kerekjarto and R. Krause, J. Med. Chem., 33 (1990) 52.
M. Takemoto and J. K. Liao, Arterioscler. Thromb. Vasc. Biol., 21 (2001) 1712.
E. Istvan, Atheroscler. Suppl., 4 (2003) 3.
A. Endo, Atheroscler. Suppl., 5 (2004) 67.
T. Wajima, S. Makita and K. Oshima, Clinical and Experimental Pharmacology and Physiology, 30 (2003) 958.
W. Aman, W. Aman and K. Thoma, Pharmazie, 58 (2003) 877.
B. Glass, Cs. Novák and M. Brown, J. Therm. Anal. Cal., 77 (2004) 1013.
T. R. Hurley, C. E. Colson, S. A. Clipper, S. E. Uhlendorf and M. D. Reily, Tetrahedron, 49 (1993) 1979.
M. W. Lam and S. A. Mabury, Aquatic Sciences — Research Across Boundaries, 67 (2005) 177.
J. Mielcarek, M. Kula, R. Zych and P. Grobelny, Reaction Kinetics and Catalysis Letters, 86 (2005) 119.
G. Miolo, S. Caffieri, L. Levorato, M. Imbesi, P. Giusti, T. Uz, R. Manev and H. Manev, Eur. J. Pharmacol., 450 (2002) 223.
B. Marciniec, M. Stawny, M. Kozak and M. Naskrent, J. Therm. Anal. Cal., 84 (2006) 741.
B. Marciniec, M. Kozak, M. Naskrent, K. Dettlaff, M. Ogrodowczyk, M. Stawny and L. Wachowski, J. Therm. Anal. Cal., 88 (2007) 337.
M. Naskrent and J. Mielcarek, Spectrochim. Acta, Part A: Molecular and Biomolecular Spectroscopy, 67 (2007) 251.
B. Marciniec, M. Stawny, M. Kozak and M. Naskrent, Spectrochimica Acta, Part A: Molecular and Biomolecular Spectroscopy, 69 (2008) 865.
K. Dettlaff, B. Teżyk, B. Marciniec, R. Wachowiak, M. Naskrent and B. Bednarek, Chemical Analysis (Warsaw), 53 (2008) 171.
B. Marciniec, M. Stawny, M. Hoffmann and M. Naskrent, J. Therm. Anal. Cal., 93 (2008) 733.
H. B. Ambroż, E. M. Kornacka, B. Marciniec, M. Ogrodowczyk and G. K. Przybytniak, Rad. Phys. Chem., 58 (2000) 357.
R. Köseoğlu, E. Köseoğlu and F. Köksal, Appl. Rad. Isotopes, 58 (2003) 63.
M. Gibella, A. S. Crucq, B. Tilquin, P. Stocker, G. Lesgards and J. Raffi, Rad. Phys. Chem., 58 (2000) 69.
A. S. Crucq, Chim. Nouv., 12 (1994) 1356.
L. Varshney and K. M. Patel, Rad. Phys. Chem., 43 (1994) 471.
N. G. S. Gopal, K. M. Patel, G. Sharma, H. L. Bhalla, P. A. Wills and N. Hilmy, Rad. Phys. Chem., 32 (1988) 619.
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Mielcarek, J., Naskrent, M. & Grobelny, P. Photochemical properties of simvastatin and lovastatin induced by radiation. J Therm Anal Calorim 96, 301–305 (2009). https://doi.org/10.1007/s10973-008-9322-6
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DOI: https://doi.org/10.1007/s10973-008-9322-6