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Thermodynamic analysis of denatured lysozyme folded on moderately hydrophobic surface at 298 K

Abstract

Both calorimetric determination of displacement adsorption enthalpies ΔH and measurement of adsorbed amounts of lysozyme (Lyz) denatured by 1.8 mol L−1 guanidine hydrochloride (GuHCl) on a moderately hydrophobic packings at 298 K, pH 7.0 and various salt concentrations were carried out. Based on the thermodynamics of stoichiometric displacement theory (SDT) the fractions of thermodynamic functions, which related to four subprocesses of denatured protein refolding on the surface, were calculated and thermodynamic analysis that which one of the subprocesses plays major role for contribution to the thermodynamic fractions was made in detail. The moderately hydrophobic surface can provide denatured Lyz energy and make it gain more conformation with surface coverage or salt concentration increment. The displacement adsorptions of denatured Lyz onto PEG-600 surface are exothermic, more structure-ordered and enthalpy driven processes.

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References

  1. 1

    W. Norde and J. P. Favier, J. Colloids Surface, 64 (1992) 87.

    Article  CAS  Google Scholar 

  2. 2

    H. Larsericsdotter, S. Oscarsson and J. Buijs, J. Colloid Interface Sci., 276 (2004) 261.

    Article  CAS  Google Scholar 

  3. 3

    C. Giancola, J. Therm. Anal. Cal., 91 (2008) 79.

    Article  CAS  Google Scholar 

  4. 4

    T. S. Norcross and T. O. Yeates, J. Mol. Biol., 362 (2006) 605.

    Article  CAS  Google Scholar 

  5. 5

    X. D. Geng and Q. Bai, Sci. China (Ser. B), 45 (2002) 655.

    Article  CAS  Google Scholar 

  6. 6

    X. D. Geng, J. Zhang and Y. M. Wei, Chin. Sci. Bull., 45 (2000) 237.

    Article  CAS  Google Scholar 

  7. 7

    F. Y. Lin, W. Y. Chen, R. C. Ruaan and H. M. Huang, J. Chromat. A, 872 (2000) 37.

    Article  CAS  Google Scholar 

  8. 8

    V. A. Lee, R. G. Craig, F. E. Filisko and R. Zand, J. Colloid Interface Sci., 288 (2005) 6.

    Article  CAS  Google Scholar 

  9. 9

    S. L. Huang, F. Y. Lin and C. P. Yang, Eur. J. Pharm. Sci., 24 (2005) 545.

    Article  CAS  Google Scholar 

  10. 10

    X. P. Geng, H. F. Zhang, B. H. Wang, X. D. Geng and J. W. Xing, J. Therm. Anal. Cal., 82 (2005) 193.

    Article  CAS  Google Scholar 

  11. 11

    X. P. Geng, Y. N. Wu, B. H. Wang, H. F. Zhang, X. D. Geng and J. W. Xing, J. Therm. Anal. Cal., 85 (2006) 601.

    Article  CAS  Google Scholar 

  12. 12

    X. P. Geng, Y. N. Wu, J. R. Song, X. D. Geng, J. W. Xing and Z. M. Lei, J. Therm. Anal. Cal., 85 (2006) 593.

    Article  CAS  Google Scholar 

  13. 13

    H. Larsericsdotter, S. Oscarsson and J. Buijs, J. Colloid Interface Sci., 237 (2001) 98.

    Article  CAS  Google Scholar 

  14. 14

    I. Haque, R. Singh, A. A. Moosavi-Movahedi and F. Ahmad, Biophys. Chem., 117 (2005) 1.

    Article  CAS  Google Scholar 

  15. 15

    X. P. Geng, M. R. Zheng, B. H. Wang, Z. M. Lei and X. D. Geng, J. Therm. Anal. Cal., 93 (2008) 503.

    Article  CAS  Google Scholar 

  16. 16

    X. P. Geng, T. S. Han and C. Cao, J. Thermal Anal., 45 (1995) 157.

    Article  CAS  Google Scholar 

  17. 17

    X. P. Geng, Thermochim. Acta, 308 (1998) 131.

    Article  CAS  Google Scholar 

  18. 18

    E. Blanco, J. M. Ruso, J. Sabin, G. Prieto and F. Sarmiento, J. Therm. Anal. Cal., 87 (2007) 211.

    Article  CAS  Google Scholar 

  19. 19

    A. Michnik, K. Michalik and Z. Drzazga, J. Therm. Anal. Cal., 80 (2005) 399.

    Article  CAS  Google Scholar 

  20. 20

    N. Brandes, P. B. Welzel, C. Werner and L. W. Kroth, J. Colloid Interface Sci., 299 (2006) 56.

    Article  CAS  Google Scholar 

  21. 21

    Z. M. Lei, X. P. Geng, L. Dai and X. D. Geng, Spectrosc. Spect. Anal., 28 (2008) 2058.

    CAS  Google Scholar 

  22. 22

    S. U. Sane, S. M. Cramer and T. M. Przybycien, J. Chromat. A, 849 (1999) 149.

    Article  CAS  Google Scholar 

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Correspondence to X. P. Geng.

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Geng, X.P., Gao, H., Wang, B.H. et al. Thermodynamic analysis of denatured lysozyme folded on moderately hydrophobic surface at 298 K. J Therm Anal Calorim 95, 345–352 (2009). https://doi.org/10.1007/s10973-008-9231-8

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Keywords

  • adsorption
  • calorimetry
  • fractions of thermodynamic functions
  • hydrophobic surface
  • lysozyme
  • subprocesses of protein folding