Abstract
The study has been undertaken to check the effect of ionising radiation on the physical and chemical properties of florfenicol, an antibiotic of a wide range of antibacterial activity. The solid-state samples were subjected to an electron beam generated by accelerator corresponding to the doses of 25, 100 and 400 kGy, and the effect of the exposure was analysed by the methods not requiring changes in the state (with no preliminary treatment), such as SEM, DSC, FTIR, XRD, EPR and HPLC.
Florfenicol irradiated with a dose of 25 kGy has not changed the form or colour, however, a small increase in intensity of some absorption bands in the FTIR spectrum and of some peaks in the XRD pattern, a decrease in the melting point by 0.6°C, the appearance of free radicals and a loss in the FF content within the error of the method (0.91%) have been observed. After irradiation with greater doses (100 and 400 kGy) the changes have intensified, yellow discolouration appeared and the loss of FF content has increased to 6.39%. As follows from the results, the compound studied in solid-state undergoes radiolysis after e-beam irradiation in the doses ≥25 kGy, but lower doses (15–20 kGy) can be applied for its decontamination or sterilization with no adverse effect on its physico-chemical properties.
Similar content being viewed by others
References
B. H. Ali, A. A. Al-Qarawi and M. Hashaad, Vet. Res. Commun., 27 (2003) 475.
X. Du, C. Xia, J. Shen, B. Wu and Z. Shen, FEMS Microbiol. Lett., 236 (2004) 183.
European Pharmacopoeia, 5th Ed., Council of Europe, Strasburg 2002.
P. Z. Zagórski, Sterylizacja radiacyjna, IChTJ, Warszawa 2007.
N. G. S. Gopal, K. M. Patel, G. Sharma, H. L. Bhalla, P. A. Wills and N. Hilmy, Radiat. Phys. Chem., 32 (1998) 619.
C. Boess and K. W. Bögl, Drug Dev. Ind. Pharm., 22 (1996) 495.
B. Marciniec, M. Stawny, E. Jaroszkiewicz and M. Needham, to be published.
CEN, 1994. EN 552. Sterilization of medical devices-validation and routine control of sterilization by irradiation. European Committee for Standardization, Rue de Strassart 36, B-1050, Belgium.
B. Marciniec, M. Stawny, M. Kozak and M. Naskrent, Spectrochim. Acta Part A: Mol. Biomol. Spectrosc., 69 (2008) 865.
B. Marciniec, M. Stawny, M. Kozak and M. Naskrent, J. Therm. Anal. Cal., 84 (2006) 741.
T. Kryczka, B. Marciniec, M. Popielarz-Brzezinska, M. Bero, J. Kasperczyk, P. Dobrzyński, Z. Kazimierczuk and P. Grieb, J. Control. Release, 89 (2003) 447.
B. Marciniec, M. Kozak and K. Dettlaff, J. Therm. Anal. Cal., 77 (2004) 305.
B. Marciniec, M. Kozak and M. Ogrodowczyk, J. Therm. Anal. Cal., 77 (2004) 581.
B. Marciniec, M. Kozak, M. Naskrent, K. Dettlaff, M. Ogrodowczyk, M. Stawny and L. Wachowski, J. Therm. Anal. Cal., 88 (2007) 337.
L. Varshney and P. B. Dodke, Radiat. Phys. Chem., 71 (2004) 1103.
S. Calis, S. Bozdag, H. S. Kas, M. Tuncay and A. A. Hincal, Il Farmaco, 57 (2002) 55.
D. Mohra, M. Wolffa and T. Kisselb, J. Control. Release, 61 (1999) 203.
L. Varshney and S. V. Choughule, Radiat. Phys. Chem., 54 (1999) 403.
L. Varshley and P. B. Dodke, Radiat. Phys. Chem., 71 (2004) 1103.
A. S. Crucq, V. Deridder and B. Tilquin, Radiat. Phys. Chem., 77 (2005) 355.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Marciniec, B., Stawny, M., Hofman, M. et al. Thermal and spectroscopic analysis of florfenicol irradiated in the solid-state. J Therm Anal Calorim 93, 733–737 (2008). https://doi.org/10.1007/s10973-008-9137-5
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10973-008-9137-5