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Microcalorimetric investigation of the growth of the Escherichia coli DH5α in different antibiotics

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Abstract

The effects of Amoxicillin Sodium and Cefuroxime Sodium on the growth of E. coli DH5α were investigated by microcalorimetry. The metabolic power-time curves of E. coli DH5α growth were determined by using a TAM air isothermal microcalorimeter at 37°C. By evaluation of the obtained parameters, such as growth rate constants (k), inhibitory ratio (I), the maximum heat power (P m) and the time of the maximum heat power (t m), one found that the inhibitory activity of Amoxicillin Sodium vs. E. coli DH5α is enhanced with the increasing of the Amoxicillin Sodium concentration, and the Cefuroxime Sodium has a stimulatory effect on the E. coli DH5α growth when the concentration is about 1 μg mL−1. The IC50 for the Amoxicillin Sodium and the Cefuroxime Sodium are 1.6 and 2.0 μg mL−1, respectively, it implicates that the E. coli DH5α is more sensitive to Amoxicillin Sodium than Cefuroxime Sodium.

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Authors and Affiliations

  1. Materials and Thermochemistry Laboratory, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian, 116023, P.R. China

    L. N. Yang, S. J. Qiu, F. Xu & L. X. Sun

  2. Graduate School of the Chinese Academy of Sciences, Beijing, 100049, P.R. China

    L. N. Yang & S. J. Qiu

  3. Division of Biotechnology, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian, 116023, P.R. China

    Z. B. Zhao

  4. Hunan Institute of Drug Detection, Changsha, 410001, P.R. China

    J. G. Liang

  5. Dalian Institute of Drug Detection, Dalian, 116021, P.R. China

    C. G. Song

Authors
  1. L. N. Yang
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  2. S. J. Qiu
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  3. F. Xu
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  4. L. X. Sun
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  5. Z. B. Zhao
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  6. J. G. Liang
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  7. C. G. Song
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Correspondence to F. Xu.

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Yang, L.N., Qiu, S.J., Xu, F. et al. Microcalorimetric investigation of the growth of the Escherichia coli DH5α in different antibiotics. J Therm Anal Calorim 89, 875–879 (2007). https://doi.org/10.1007/s10973-006-7902-x

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  • DOI: https://doi.org/10.1007/s10973-006-7902-x

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