Abstract
A microcalorimetric technique based on the bacterial heat output was applied to evaluate the influence of antibiotics PIP (Piperacillin Sodium) and composite preparation of PIP and SBT (Sulbactam Sodium) on the growth of E. coli DH5α. The power–time curves of the growth metabolism of E. coli DH5α were studied using a TAM Air Isothermal Microcalorimeter at 37°C. By analyzing the power–time curves, the parameters such as growth rate constants (k), inhibitory ratio (I), the maximum heat power (P m) and the time of the maximum heat power (t m) were obtained. The results show that different concentrations of antibiotics affect the growth metabolism of E. coli DH5α. The PIP in the concentration range of 0–0.05 µg mL–1 has a stimulatory effect on the E. coli DH5α growth, while the PIP of higher concentrations (0.05 –0.25 µg mL–1) can inhibit its growth. It seems that the composite preparation composed of PIP and SBT cannot improve the inhibitory effect on E. coli DH5α as compared with the PIP.
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References
A Schmalreck A Wildfeuer (1990) Drug Res. 40 1145 Occurrence Handle1:CAS:528:DyaK3MXitVyqtA%3D%3D
T Yamamoto T Tanida E Ueta T Kimura S Doi T Osaki (2001) Oral Oncol. 37 471 Occurrence Handle1:CAS:528:DC%2BD3MXjslCis78%3D Occurrence Handle10.1016/S1368-8375(00)00108-1
H Mahgoub FA Aly (1998) J. Pharm. Biomed. Anal. 17 1273 Occurrence Handle1:CAS:528:DyaK1cXmsFamtLs%3D Occurrence Handle10.1016/S0731-7085(97)00273-2
S Yurus T Ozvey M Korkmaz (2004) J. Pharm. Biomed. Anal. 35 971 Occurrence Handle1:CAS:528:DC%2BD2cXntFSmtro%3D Occurrence Handle10.1016/j.jpba.2004.03.012
SL Huang FY Lin CP Yang (2005) Eur. J. Pharm. Sci. 24 545 Occurrence Handle1:CAS:528:DC%2BD2MXisFGktrg%3D Occurrence Handle10.1016/j.ejps.2005.01.003
Z Heng Z Congyi W Cunxin W Jinbin G Chaojiang L Jie L Yuwen (2005) J. Therm. Anal. Cal. 79 45 Occurrence Handle1:CAS:528:DC%2BD2MXhs1agsr8%3D Occurrence Handle10.1007/s10973-004-0560-y
JC Zhu A Abderrahmane Y Liu P Shen Y Yang SS Qu (2005) Thermochim. Acta 439 52 Occurrence Handle1:CAS:528:DC%2BD2MXht1GlsrbF Occurrence Handle10.1016/j.tca.2005.08.037
Y Yang Y Liu J Zhu MJ Li P Shen (2005) J. Therm. Anal. Cal. 79 645 Occurrence Handle1:CAS:528:DC%2BD2MXisFyns7k%3D Occurrence Handle10.1007/s10973-005-0590-0
L Wadsö F Gomez I Sjöholm P Rocculi (2004) Thermochim. Acta 422 89 Occurrence Handle10.1016/j.tca.2004.04.031
I Wadsö (2002) Thermochim. Acta 394 305 Occurrence Handle10.1016/S0040-6031(02)00263-0
A Abderrahmane L Yi GW Ying S Ping QS Sheng (2002) J. Therm. Anal. Cal. 68 909 Occurrence Handle1:CAS:528:DC%2BD38XkvF2htLk%3D Occurrence Handle10.1023/A:1016138505617
MLH Gruwel C Alves JN Schrader (1995) Am. J. Physiol. 268 H351 Occurrence Handle1:CAS:528:DyaK2MXktFWns7w%3D
O Culic MLH Gruwel J Schrader (1997) Am. J. Physiol. 273 C205 Occurrence Handle1:CAS:528:DyaK2sXlt1Srsr4%3D
F. Xu, L. X. Sun, Z. C. Tan, J. G. Liang, C. G. Song and T. Zhang, 4th Internationanl and 6th Japan-China Joint Symposium on Calorimetry and Thermal Analysis, Fukuoka, Japan 2005, p. 170.
N Barros S Feijóo A Simoni SAM Critter C Airoldi (2001) J. Therm. Anal. Cal. 63 577 Occurrence Handle1:CAS:528:DC%2BD3MXisFWis7g%3D Occurrence Handle10.1023/A:1010162425574
F Postollec W Norde HC Mei HJ Busscher (2003) J. Microbiol. Methods 55 241 Occurrence Handle10.1016/S0167-7012(03)00145-3
SM Mcgulnness BG Barisas (1991) Environ. Sci. Technol. 25 1092 Occurrence Handle10.1021/es00018a012
L Nunez N Barros I Barja (1994) Thermochim. Acta 237 73 Occurrence Handle1:CAS:528:DyaK2cXkslyjsLg%3D Occurrence Handle10.1016/0040-6031(94)85185-9
R Holzel C Motzkus I Lamprecht (1994) Thermochim. Acta 239 17 Occurrence Handle10.1016/0040-6031(94)87052-7
X Li Y Liu RM Zhao J Wu SS Qu (2000) Biol. Trace Elem. Res. 75 167 Occurrence Handle1:CAS:528:DC%2BD3cXlt1ahsr4%3D Occurrence Handle10.1385/BTER:75:1-3:167
CL Xie HK Tang SS Qu (1988) Thermochim. Acta 123 33 Occurrence Handle10.1016/0040-6031(88)80007-8
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Yang, L.N., Xu, F., Sun, L.X. et al. Study on interaction between antibiotics and Escherichia coli DH5α by microcalorimetric method. J Therm Anal Calorim 85, 807–810 (2006). https://doi.org/10.1007/s10973-006-7507-4
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DOI: https://doi.org/10.1007/s10973-006-7507-4