Abstract
The metabolic process of BHK-21 cell line infected by foot and mouth disease virus (FMDV) was determined by using LKB-2277 Bioactivity Monitor. The aim of the present study is to investigate the metabolic thermal power of the virus infection process of BHK-21 cells, the effects of combinational treatments of hyperthermia and 1b recombinant homo interferon on this process. In contrast to the metabolic thermal power of uninfected BHK-21 cells, the thermogenetic curves show that the energy metabolism mechanisms of BHK-21 cells were significantly changed by the virus infection process. The maximum thermal power decreased and the time needed to reach the maximal thermal power increased with the increasing concentration of interferon. The results also show that the infection process was thermosensitive. But no apparent synergetic effect of the combinational treatments of hyperthermia and interferon was observed. The present microcalorimetric results are in accordance with the cytomorphology observations.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
D. Esteban, B. Eric, E. Cristina and S. Francisco, Comp. Immunol. Microb., 25 (2002) 297.
E. H. Battley, J. Therm. Anal. Cal., 74 (2003) 709.
R. B. Kemp, Handbook of Thermal Analysis and Calorimetry, Vol. 4, From Macromolecules to Man, Elsevier, Amsterdam 1999.
X.Y. Xie, C.X. Wang and Z.Y. Wang, J. Therm. Anal. Cal., 76 (2004) 275.
H. Aki, J. Therm. Anal. Cal., 68 (2002) 553.
B. Chardin, P. Gallice, J. C. Sari and M. Bruschi, J. Therm. Anal. Cal., 70 (2002) 475.
R. B. Kemp, Thermochim. Acta, 193 (1991) 253.
J. Nittinger and P. Fürst, Thermochim. Acta, 251 (1995) 155.
R. B. Kemp and Y. Guan, Thermochim. Acta, 332 (1999) 203.
Y. Xie, H. K. Joseph, W. Depierre and L. Nassberger, J. Pharmacol. Toxicol. Methods, 40 (1998) 137.
A. M. Tan, B. Xu, S. Q. Huang and S. S. Qu, Thermochim. Acta, 333 (1999) 99.
K. Ijungholm, I. Wadsö and L. Kjellen, Acta Path. Microbial. Scand. Sect. B, 86 (1978) 121.
A. M. Tan and J. H. Lu, J. Biochem. Biophys. Methods, 38 (1999) 225.
J. Suurkuusk and I. Wadsö, Chem. Scr., 20 (1982) 155.
P. Backman, T. Kimura, A. Schon and I. Wadsö, J. Cell Physiol., 150 (1992) 99.
E. K. Wagner and M. J. Hewlett, Basic Virology, Blackwell Science Inc., Berlin 1999.
A. Isaacs and J. Lindenmann, Proc. R. Soc. Lond. B., 147 (1957) 258.
I. R. Tizard, The biology of veterinary cytokines interferons. In: M. J. Myers and M. P. Murtablgh (Eds), Cytokines in Animal Health and Disease. Marcel Dekker Inc, New York 1995.
G. J. Michael and G. K. Michael, Pharmacol. Therapeut., 78 (1998) 29.
J. P. Mather and P. E. Roberts, Introduction to Cell and Tissue Culture: Theory and Technique, Plenum Press, New York 1998.
P. Lönnbro and A. Schön, Thermochim. Acta, 172 (1990) 75.
Y. Feng, Z. F. Luo, S. S. Qu, C. Y. Zheng and H. Xu, Thermochim. Acta, 303 (1997) 203.
L. S. Geoffrey, A. S. Julian and A. Antonio, Sem. Virol., 8 (1998) 409.
H. Robins, W. L. I. Longo, R. A. Steeves, Lagoni, K. Rhonda, A. J. H. Anders, M. Ch. B. Neville, S. O’Keefe, W. Giese and C. L. Schmitt, Int. J. Radiation Oncology Biol. Phys., 15 (1988) 427.
J. van der Zee, D. G. Gonzalez, G. C. van Rhoon, J. D. P. van Dijk, W. L. J. van Putten and A. A. M. Hart, The Lancet, 355 (2000) 1119.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Heng, Z., Congyi, Z., Cunxin, W. et al. Microcalorimetric study of virus infection; The effects of hyperthermia and 1b recombinant homo interferon on the infection process of BHK-21 cells by foot and mouth disease virus. J Therm Anal Calorim 79, 45–50 (2005). https://doi.org/10.1007/s10973-004-0560-y
Issue Date:
DOI: https://doi.org/10.1007/s10973-004-0560-y