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Green and chemical approach for synthesis of Ag2O nanoparticles and their antimicrobial activity


Green synthesis of nanomaterials is a current topic of extensive research and a number of green approaches as microorganisms and plant extracts have been explored as an alternative method for their synthesis. In this current work, an attempt has been made to observe the properties of silver oxide nanoparticles (Ag2O NPs), chemically prepared from silver ammonia complex by the traditional sol-gel method using citric acid, as well as from a green approach using aqueous leaf extract of Salix Integra. The synthesized Ag2O NPs were characterized by Powder X-ray Diffraction technique (PXRD), X-ray Photoelectron Spectroscopy (XPS), Scanning Electron Microscope (SEM), UV-Visible spectroscopy, Transmission electron microscope (TEM) and Fourier-Transform Infrared Spectroscopy (FT-IR). PXRD analysis showed that, the average particle size of the Ag2O NPs is 17.5 nm and 27.5 nm respectively from the chemical and the green approach. Green approach based Ag2O NPs and Salix Integra leaf extract are tested against gram-negative (E. coli) and gram-positive (S. aureus). In addition the Ag2O Nps were found to be effective against different microbial strains like M. tuberculosis H37Rv, M. Chelonae, M. Abscessus, and M. Fortuitum.

Graphical Abstract

The successful synthesis of Ag2O NPs by green and chemical approached and their use as a bactericidal agent against different microbes.


  • Synthesis of Ag2O NP’s using green and chemical methods from Silver Ammonia Complex.

  • Characterization and comparative studies of the properties of the prepared Ag2O NP’s.

  • Studies for the bioapplication of Ag2O NP’s for antimicrobial studies.

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  1. Vinay SP, Chandrasekhar N, Bhanu U, et al. (2019) Ixora coccinea extract-mediated green synthesis of silver nanoparticles: photodegradative and antimicrobial studies. Int J Biosensors Bioelectronics 5:

  2. Ueda N, Maeda H, Hosono H, Kawazoe H (1998) Band-gap widening of CdO thin films. J Appl Phys 84:6174–6177.

    Article  CAS  Google Scholar 

  3. Liu H, Zhang X, Li L, et al. (2007) Role of point defects in room-temperature ferromagnetism of Cr-doped ZnO. Appl Phys Lett 91:

  4. Maheshwaran G, Nivedhitha Bharathi A, Malai Selvi M, et al. (2020) Green synthesis of Silver oxide nanoparticles using Zephyranthes Rosea flower extract and evaluation of biological activities. J Environ Chem Eng 8:104137.

    Article  CAS  Google Scholar 

  5. Khan I, Saeed K, Khan I (2019) Nanoparticles: Properties, applications and toxicities. Arab J Chem 12:908–931

    Article  CAS  Google Scholar 

  6. Vaseashta A, Dimova-Malinovska D (2005) Nanostructured and nanoscale devices, sensors and detectors. In: Science and Technology of Advanced Materials. pp 312–318

  7. Liang Z, Zhao J, Zhang Y (2010) Palladium-catalyzed regioselective oxidative coupling of indoles and one-pot synthesis of acetoxylated biindolyls. J Org Chem 75:170–177.

    Article  CAS  Google Scholar 

  8. Mabrook M, Hawkins P (2001) A rapidly-responding sensor for benzene, methanol and ethanol vapours based on films of titanium dioxide dispersed in a polymer operating at room temperature. Sensors and Actuators B-chemical - SENSOR ACTUATOR B-CHEM. 75:197–202.

  9. Ferreira FF, Tabacniks MH, Fantini MCA, et al. (1996) Electrochromic nickel oxide thin films deposited under different sputtering conditions. Solid State Ion 86–88:971–976.

    Article  Google Scholar 

  10. Shume WM, Murthy HCA, Zereffa EA (2020) A Review on Synthesis and Characterization of Ag2O Nanoparticles for Photocatalytic Applications. J Chem 2020.

  11. Seil JT, Webster TJ (2012) Antibacterial effect of zinc oxide nanoparticles combined with ultrasound. Nanotechnology 23:

  12. Alivov YI, Kalinina EV, Cherenkov AE, et al (2003) Fabrication and characterization of n-ZnO/p-AlGaN heterojunction light-emitting diodes on 6H-SiC substrates. Appl Phys Lett 83:4719–4721.

    Article  CAS  Google Scholar 

  13. Lallo da Silva B, Caetano BL, Chiari-Andréo BG, et al (2019) Increased antibacterial activity of ZnO nanoparticles: Influence of size and surface modification. Colloids Surf B: Biointerfaces 177:440–447.

    Article  CAS  Google Scholar 

  14. Iqbal S, Fakhar-e-Alam M, Akbar F, et al (2019) Application of silver oxide nanoparticles for the treatment of cancer. J Mol Struct 1189:203–209.

    Article  CAS  Google Scholar 

  15. Skiba MI, Vorobyova VI, Pivovarov A, Makarshenko NP (2020) Green synthesis of silver nanoparticles in the presence of polysaccharide: Optimization and characterization. J Nanomater 2020:

  16. Panáček A, Kvítek L, Prucek R et al. (2006) Silver colloid nanoparticles: Synthesis, characterization, and their antibacterial activity. J Phys Chem B 110:16248–16253.

    Article  CAS  Google Scholar 

  17. Flores-Lopez NS, Cervantes-Chávez JA, Téllez de Jesús DG, et al. (2021) Bactericidal and fungicidal capacity of Ag2O/Ag nanoparticles synthesized with Aloe vera extract. J Environ Sci Health - Part A Toxic/Hazard Substances Environ Eng 56:762–768.

    Article  CAS  Google Scholar 

  18. Singh J, Dutta T, Kim KH, et al. (2018) “Green” synthesis of metals and their oxide nanoparticles: Applications for environmental remediation. J Nanobiotechnology 16:1–24.

    Article  CAS  Google Scholar 

  19. Fayyadh AA, Jaduaa Alzubaidy MH (2021) Green-synthesis of Ag2O nanoparticles for antimicrobial assays. J Mech Behav Mater 30:228–236.

    Article  Google Scholar 

  20. Aravind M, Ahmad A, Ahmad I, et al. (2021) Critical green routing synthesis of silver NPs using jasmine flower extract for biological activities and photocatalytical degradation of methylene blue. J Environ Chem Eng 9:104877.

    Article  CAS  Google Scholar 

  21. Thangavelu S, Dhandapani R, Arulprakasam A, et al. (2022) Isolation, Identification, Characterization, and Plasmid Profile of Urinary Tract Infectious Escherichia coli from Clinical Samples. Evidence-based Complementary and Alternative Med 2022:

  22. Sharma AK, Dhasmana N, Dubey N, et al. (2017) Bacterial Virulence Factors: Secreted for Survival. Indian J Microbiol 57:1–10.

    Article  Google Scholar 

  23. Behra PRK, Das S, Pettersson BMF, et al. (2019) Extended insight into the Mycobacterium chelonae-abscessus complex through whole genome sequencing of Mycobacterium salmoniphilum outbreak and Mycobacterium salmoniphilum-like strains. Scientific Rep 9.

  24. Chandini KM, Al-Ostoot FH, Shehata EE, et al. (2021) Synthesis, crystal structure, Hirshfeld surface analysis, DFT calculations, 3D energy frameworks studies of Schiff base derivative 2,2′-((1Z,1′Z)-(1,2-phenylene bis(azanylylidene)) bis(methanylylidene)) diphenol. J Mol Struct 1244:

  25. Elemike EE, Onwudiwe DC, Ekennia AC, et al. (2017) Green synthesis of Ag/Ag2O nanoparticles using aqueous leaf extract of Eupatorium odoratum and its antimicrobial and mosquito larvicidal activities. Molecules 22:1–15.

    Article  CAS  Google Scholar 

  26. Lu PL, Liu YC, Toh HS, et al. (2012) Epidemiology and antimicrobial susceptibility profiles of Gram-negative bacteria causing urinary tract infections in the Asia-Pacific region: 2009–2010 results from the Study for Monitoring Antimicrobial Resistance Trends (SMART). Int J Antimicrob Agents 40:

  27. Ravichandran S, Paluri V, Kumar G, et al. (2016) A novel approach for the biosynthesis of silver oxide nanoparticles using aqueous leaf extract of Callistemon lanceolatus (Myrtaceae) and their therapeutic potential. J Exp Nanosci 11:445–458.

    Article  CAS  Google Scholar 

  28. Wei W, Mao X, Ortiz LA, Sadoway DR (2011) Oriented silver oxide nanostructures synthesized through a template-free electrochemical route. J Mater Chem 21:432–438.

    Article  CAS  Google Scholar 

  29. Sun Q, Cai X, Li J, et al. (2014) Green synthesis of silver nanoparticles using tea leaf extract and evaluation of their stability and antibacterial activity. Colloids Surf A: Physicochemical Eng Asp 444:226–231.

    Article  CAS  Google Scholar 

  30. Jain S, Mehata MS (2017) Medicinal plant leaf extract and pure flavonoid mediated green synthesis of silver nanoparticles and their enhanced antibacterial property. Sci Rep 7:1–14.

    Article  CAS  Google Scholar 

  31. Armani MA, Abu-Taleb A, Remalli N, et al. (2016) Dragon’s blood-aided synthesis of Ag/Ag2O core/shell nanostructures and Ag/Ag2O decked multi-layered graphene for efficient As(III) uptake from water and antibacterial activity. RSC Adv 6:44145–44153.

    Article  CAS  Google Scholar 

  32. Albiter E, Valenzuela MA, Alfaro S, et al. (2015) Photocatalytic deposition of Ag nanoparticles on TiO2: Metal precursor effect on the structural and photoactivity properties. J Saudi Chem Soc 19:563–573.

    Article  Google Scholar 

  33. Zielińska A, Kowalska E, Sobczak JW, et al. (2010) Silver-doped TiO2 prepared by microemulsion method: Surface properties, bio- and photoactivity. Sep Purif Technol 72:309–318.

    Article  CAS  Google Scholar 

  34. Mani M, Harikrishnan R, Purushothaman P, et al. (2021) Systematic green synthesis of silver oxide nanoparticles for antimicrobial activity. Environ Res 202:

  35. Chinnaraj S, Palani V, Maluventhen V, et al. (2022) Silver nanoparticle production mediated by Goniothalamus wightii extract: characterization and their potential biological applications. Particulate Sci Technol 0:1–15.

    Article  CAS  Google Scholar 

  36. Mulvaney P (1996) Surface plasmon spectroscopy of nanosized metal particles. Langmuir 12:788–800.

    Article  CAS  Google Scholar 

  37. Gole A, Dash C, Ramakrishnan V, et al. (2001) Pepsin-gold colloid conjugates: Preparation, characterization, and enzymatic activity. Langmuir 17:1674–1679.

    Article  CAS  Google Scholar 

  38. Vinay SP, Udayabhanu, Nagaraju G, et al. (2019) Rauvolfia tetraphylla (Devil Pepper)-Mediated Green Synthesis of Ag Nanoparticles: Applications to Anticancer, Antioxidant and Antimitotic. J Clust Sci 30:1545–1564.

    Article  CAS  Google Scholar 

  39. Allahverdiyev AM, Abamor ES, Bagirova M, Rafailovich M (2011) Antimicrobial effects of TiO(2) and Ag(2)O nanoparticles against drug-resistant bacteria and leishmania parasites. Future Microbiol 6:933–940

    Article  CAS  Google Scholar 

  40. Neuberger T, Schöpf B, Hofmann H, et al. (2005) Superparamagnetic nanoparticles for biomedical applications: Possibilities and limitations of a new drug delivery system. In: J Magnetism Magnetic Mater 293:483–496

  41. Vinay SP, Udayabhanu, Nagaraju G, et al. (2019) Novel Gomutra (cow urine) mediated synthesis of silver oxide nanoparticles and their enhanced photocatalytic, photoluminescence and antibacterial studies. J Sci: Adv Mater Devices 4:392–399.

    Article  Google Scholar 

  42. Kumar R, Ashfaq M, Verma N (2018) Synthesis of novel PVA–starch formulation-supported Cu–Zn nanoparticle carrying carbon nanofibers as a nanofertilizer: controlled release of micronutrients. J Mater Sci 53:7150–7164.

    Article  CAS  Google Scholar 

  43. Kaviya S, Santhanalakshmi J, Viswanathan B, et al. (2011) Biosynthesis of silver nanoparticles using citrus sinensis peel extract and its antibacterial activity. Spectrochimica Acta - Part A: Mol Biomolecular Spectrosc 79:594–598.

    Article  CAS  Google Scholar 

  44. Indumathy R, Sreeram KJ, Sriranjani M, et al. (2010) Bifunctional role of Thiosalicylic Acid in the synthesis of Silver nanoparticles. Mater Sci Appl 01:272–278.

    Article  CAS  Google Scholar 

  45. Hosseinpour-Mashkani SM, Ramezani M (2014) Silver and silver oxide nanoparticles: Synthesis and characterization by thermal decomposition. Mater Lett 130:259–262.

    Article  CAS  Google Scholar 

  46. Urnukhsaikhan E, Bold BE, Gunbileg A, et al. (2021) Antibacterial activity and characteristics of silver nanoparticles biosynthesized from Carduus crispus. Sci Rep 11:1–12.

    Article  Google Scholar 

  47. Sood R, Chopra DS (2018) Optimization of reaction conditions to fabricate Ocimum sanctum synthesized silver nanoparticles and its application to nano-gel systems for burn wounds. Mater Sci Eng C 92:575–589.

    Article  CAS  Google Scholar 

  48. Akhavan O, Ghaderi E (2010) Self-accumulated Ag nanoparticles on mesoporous TiO2 thin film with high bactericidal activities. Surf Coat Technol 204:3676–3683.

    Article  CAS  Google Scholar 

  49. Calamak S, Aksoy EA, Ertas N, et al. (2015) Ag/silk fibroin nanofibers: Effect of fibroin morphology on Ag+ release and antibacterial activity. Eur Polym J 67:99–112.

    Article  CAS  Google Scholar 

  50. Guo Y, Wang S, Du H, et al. (2019) Silver Ion-Histidine Interplay Switches Peptide Hydrogel from Antiparallel to Parallel β-Assembly and Enables Controlled Antibacterial Activity. Biomacromolecules 20:558–565.

    Article  CAS  Google Scholar 

  51. Yin IX, Zhang J, Zhao IS, et al. (2020) The antibacterial mechanism of silver nanoparticles and its application in dentistry. Int J Nanomed 15:2555–2562.

    Article  CAS  Google Scholar 

  52. Xiu Z, Ma J, Alvarez PJJ (2011) Differential effect of common ligands and molecular oxygen on.pdf. Environ Sci Technol 45:9003–9008

    Article  CAS  Google Scholar 

  53. Shanmuganathan R, MubarakAli D, Prabakar D, et al. (2018) An enhancement of antimicrobial efficacy of biogenic and ceftriaxone-conjugated silver nanoparticles: Green approach. Environ Sci Pollut Res 25:10362–10370.

    Article  CAS  Google Scholar 

  54. Zhang J, Liu H, Ma Z (2016) Flower-like Ag2O/Bi2MoO6 p-n heterojunction with enhanced photocatalytic activity under visible light irradiation. J Mol Catal A: Chem 424:37–44.

    Article  CAS  Google Scholar 

  55. Murugappan A, Sudarsan JS, Manoharan A (2006) Effects of using Lignite mine drainage for irrigation on soils - A case study of perumal tank command area in Tamilnadu State. J Ind Pollut Control 22:149–160

    CAS  Google Scholar 

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We gratefully acknowledge Malaviya National Institute of Technology Jaipur, India (all experimental work, TEM, SEM, and FT-IR studies) and Central University of Rajasthan, India (PXRD). We also acknowledge Dr. Sonkar S.K. (UV‐Vis study) for their invaluable aid. And our special thanks CSIR-Central Drug Research Institute, Lucknow, India (Antimicrobial activity test). We thankful to APX LABORATORIES Mumbai for antibacterial activity test.

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Patel, H., Joshi, J. Green and chemical approach for synthesis of Ag2O nanoparticles and their antimicrobial activity. J Sol-Gel Sci Technol 105, 814–826 (2023).

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