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  • Original Paper: Sol-gel and hybrid materials for catalytic, photoelectrochemical and sensor applications
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Characterization of the catalytic ability and surface properties of a heterogeneous biocatalyst obtained by the sol-gel method


A heterogeneous biocatalyst was obtained by immobilizing the cells of the yeast Debaryomyces hansenii VKM Y-2482 and the bacteria Paracoccus yeei VKM B-3302 into an organosilica material using the sol-gel method. The catalytic activity of immobilized cells was characterized using a heterogeneous biocatalyst as a bioreceptor element of the biosensor. The surface properties of the synthesized material were studied using the BET and BJH methods. It was shown that a heterogeneous biocatalyst with a composition of 50 vol.% MTES and 50 vol.% TEOS exhibited the highest catalytic efficiency. The formed material was mesoporous with slit-like pores. It was shown that the BOD-biosensor with the developed heterogeneous biocatalyst allows data to be obtained that were consistent with the certified method for determining the BOD.

Graphical abstract


  • Immobilizing together bacteria Paracoccus yeei and yeast Debaryomyces hansenii.

  • Formation of material from methyltriethoxysilane and tetraethoxysilane.

  • The biosensor approach for catalytic activity measures immobilized cells.

  • Brunauer–Emmett–Teller and Barrett–Joyner–Halenda methods for the analysis of the surface.

  • Heterogeneous biocatalyst for determining biochemical oxygen demand.

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This work was financially supported by the Ministry of Science and Higher Education of the Russian Federation within the framework of a state assignment on the topic “Synthesis of targeted biologically active ionic compounds and new biocomposite materials” (FEWG-2021-0011).

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Correspondence to O. A. Kamanina.

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Rybochkin, P.V., Kamanina, O.A., Lantsova, E.A. et al. Characterization of the catalytic ability and surface properties of a heterogeneous biocatalyst obtained by the sol-gel method. J Sol-Gel Sci Technol (2022).

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  • Sol-gel
  • Methyltriethoxysilane
  • Yeast
  • Bacteria
  • Tetraethoxysilane
  • Biosensor