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In vitro antibacterial property assessment of silver nanoparticles synthesized by Falcaria vulgaris aqueous extract against MDR bacteria

  • Original Paper: Nano-structured materials (particles, fibers, colloids, composites, etc.)
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Abstract

Silver nanoparticles (AgNPs) were fabricated in the presence of Falcaria vulgaris aqueous extract as a biosynthesis method without utilizing any surfactant or template. AgNPs were prepared under different synthesis conditions such as silver ion concentration and the amount of plant used for the extraction, reaction duration and temperature for the extraction. The effect of these variables on the size of resulted AgNPs was examined, and operation conditions were optimized statistically with analysis of variance (ANOVA) to describe the role of these variables in tuning the size of AgNPs. The results of ANOVA displayed the optimum conditions for the synthesis procedure that resulted in AgNPs with the average size of 28 ± 8 nm. Furthermore, the growth of AgNPs was monitored by UV-Vis spectroscopy, and they were characterized using TEM, SEM, X-ray diffraction, and FT-IR spectroscopy. Finally, in vitro antibacterial activity of the AgNPs showed the maximum inhibition zone alongside Staphylococcus aureus (ATCC 25923) and lowermost inhibition zone touching E. coli (MDR). The minimum inhibitory concentration (MIC) for the AgNP-Fv was in a range between 0.535 and 0.001 µg/ml. According to the results, the ATCC bacteria were more sensitive to AgNP-Fv compared to multiple-drug resistance bacteria, except for Pseudomonas aeruginosa (MDR).

Highlights

  • Ag nanoparticles were synthesized by a green procedure.

  • Aqueous extract of Falcaria vulgaris was used to synthesize Ag nanoparticles.

  • Taguchi statistical design was used to optimize the synthesis procedure.

  • Ag nanoparticles were characterized by UV-Vis, EDX, SEM, TEM, and FT-IR.

  • In vitro antibacterial activity of Ag-NPs was evaluated against MDR bacteria.

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Authors and Affiliations

  1. Faculty of Chemistry and Chemical Engineering, Malek Ashtar University of Technology, Tehran, Iran

    Iraj Kohsari, Masoumeh Foroutan Koudehi & Seied Mahdi Pourmortazavi

  2. Department of Physiology and Pharmacology, School of Medicine, Sabzevar University of Medical Sciences, Sabzevar, Iran

    Mohammad Mohammad-Zadeh

  3. Antimicrobial Resistance Research Center, Institute of Immunology and Infectious Diseases, Iran University of Medical Sciences, Tehran, Iran

    Sara Minaeian

  4. Department of Chemistry, Amirkabir University of Technology (Tehran Polytechnic), PO Box 15875-4413, Tehran, Iran

    Morteza Rezaee, Azam Barzegari & Zahra Shariatinia

  5. Endocrinology and Metabolism Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences, 1411713137, Tehran, Iran

    Somayeh Mirsadeghi

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  1. Iraj Kohsari
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Correspondence to Mohammad Mohammad-Zadeh, Somayeh Mirsadeghi or Seied Mahdi Pourmortazavi.

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Kohsari, I., Mohammad-Zadeh, M., Minaeian, S. et al. In vitro antibacterial property assessment of silver nanoparticles synthesized by Falcaria vulgaris aqueous extract against MDR bacteria. J Sol-Gel Sci Technol 90, 380–389 (2019). https://doi.org/10.1007/s10971-019-04961-0

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