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Preparation and property of layer-by-layer alginate hydrogel beads based on multi-phase emulsion technique

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Abstract

Layer-by-layer (LbL) alginate beads, which were prepared by multi-phase emulsion technique, had been fabricated via the ionic crosslinking between calcium ion (Ca2+) and the carboxylic group of alginate. The prepared beads were spherical, smooth-surfaced and non-aggregated. The SEM analysis displayed the LbL structure of the beads clearly. It had been demonstrated that the size of the beads was controllable and had a correlation with concentration of sodium alginate (SA) and CaCl2, ratio of water phase and oil phase (W/O), stirring speed, pH value of the water phase, viscosity of SA as well as the temperature for solidification. Stability studies showed that the beads degraded slowly in simulated gastric fluid and simulated intestinal fluid but degraded sharply when they were moved to simulated colonic fluid. Cytotoxicity study by MTT assay indicated that the prepared beads are slightly toxic. It is hoped that this kind of novel beads could be used in pharmaceutical area and cell culture area.

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Acknowledgments

This work was supported by grants from the National Natural Science Foundation of China (NSFC,81071274), International S&T Cooperation Program of China (ISTCP,2011DFA31270).

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Authors and Affiliations

  1. College of Marine Life Science, Ocean University of China, 5# Yushan Road, Qingdao, 266003, People’s Republic of China

    Yang Li, Ming Kong, Chao Feng, Wei Fang Liu, Ya Liu, Xiao Jie Cheng & Xi Guang Chen

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  1. Yang Li
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  2. Ming Kong
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  3. Chao Feng
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  4. Wei Fang Liu
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  5. Ya Liu
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  6. Xiao Jie Cheng
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  7. Xi Guang Chen
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Correspondence to Xiao Jie Cheng or Xi Guang Chen.

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Li, Y., Kong, M., Feng, C. et al. Preparation and property of layer-by-layer alginate hydrogel beads based on multi-phase emulsion technique. J Sol-Gel Sci Technol 62, 217–226 (2012). https://doi.org/10.1007/s10971-012-2712-z

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  • DOI: https://doi.org/10.1007/s10971-012-2712-z

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