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Catalytic activity and stability of laccase entrapped in sol–gel silica with additives

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Abstract

This study investigated the effects of different additives and precursors on the catalytic activity of laccase entrapped in sol–gel silica. It was found that the laccase catalytic activity and stability of sol–gel laccase could be enhanced if the entrapment was performed in the presence of additives such as PVA, PEG and APTS. The use of TEOS as a precursor showed slightly higher laccase catalytic activity compared to TMOS. The PVA as an additive showed a better catalytic activity enhancement compared to the PEG and APTMS with the optimum PVA concentration of 0.03 mg/mL. The optimal temperatures of sol–gel laccase without and with additives were found to be at 40 and 27°C, respectively. After 70 days of storage at 27°C, the catalytic activity of the immobilized sol–gel laccase with additives maintained its catalytic activity compared to only 30% of its original catalytic activity for the sol–gel laccase without additives.

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Acknowledgments

The authors would like to acknowledge the financial support from the Ministry of Higher Education (MOHE), Malaysia, under the Fundamental Research Grant Scheme (FRGS Project No. 78213). The assistance given by Mrs. Siti Zalita in the analytical work is appreciated.

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Authors and Affiliations

  1. Advanced Materials and Process Engineering Laboratory, Faculty of Chemical Engineering, Universiti Teknologi Malaysia, UTM Skudai, 81310, Johor, Malaysia

    Nur Atikah Mohidem

  2. Advanced Materials and Process Engineering Laboratory (Faculty of Chemical Engineering), Novel Materials Research Group, Nanotechnology Research Alliance, Universiti Teknologi Malaysia, UTM Skudai, 81310, Johor, Malaysia

    Hanapi Bin Mat

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  1. Nur Atikah Mohidem
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Correspondence to Hanapi Bin Mat.

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Mohidem, N.A., Bin Mat, H. Catalytic activity and stability of laccase entrapped in sol–gel silica with additives. J Sol-Gel Sci Technol 61, 96–103 (2012). https://doi.org/10.1007/s10971-011-2596-3

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  • DOI: https://doi.org/10.1007/s10971-011-2596-3

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