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The impact of structural genomics: the first quindecennial

  • Published:
Journal of Structural and Functional Genomics

Abstract

The period 2000–2015 brought the advent of high-throughput approaches to protein structure determination. With the overall funding on the order of $2 billion (in 2010 dollars), the structural genomics (SG) consortia established worldwide have developed pipelines for target selection, protein production, sample preparation, crystallization, and structure determination by X-ray crystallography and NMR. These efforts resulted in the determination of over 13,500 protein structures, mostly from unique protein families, and increased the structural coverage of the expanding protein universe. SG programs contributed over 4400 publications to the scientific literature. The NIH-funded Protein Structure Initiatives alone have produced over 2000 scientific publications, which to date have attracted more than 93,000 citations. Software and database developments that were necessary to handle high-throughput structure determination workflows have led to structures of better quality and improved integrity of the associated data. Organized and accessible data have a positive impact on the reproducibility of scientific experiments. Most of the experimental data generated by the SG centers are freely available to the community and has been utilized by scientists in various fields of research. SG projects have created, improved, streamlined, and validated many protocols for protein production and crystallization, data collection, and functional analysis, significantly benefiting biological and biomedical research.

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Acknowledgments

We would like to thank Steve Burley, Andrzej Joachimiak, Maks Chruszcz, Gaetano Montelione, Wayne Anderson, Steve Almo, Torsten Schwede, Alex Wlodawer, Zbyszek Dauter and David Cooper for reading the manuscripts and helpful discussion. The authors’ research was supported with federal funds from the National Institute of Allergy and Infectious Diseases, National Institutes of Health, Department of Health and Human Services, under Contract No. HHSN272201200026C, by NIH Grants HG008424, GM093342, GM094585, GM093324 and GM094662.

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    1. Department of Molecular Physiology and Biological Physics, University of Virginia School of Medicine, 1340 Jefferson Park Avenue, Jordan Hall, Room 4223, Charlottesville, VA, 22908, USA

      Marek Grabowski, Ewa Niedzialkowska, Matthew D. Zimmerman & Wladek Minor

    2. Jerzy Haber Institute of Catalysis and Surface Chemistry, Polish Academy of Sciences, Niezapominajek 8, 30-239, Kraków, Poland

      Ewa Niedzialkowska

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    Correspondence to Wladek Minor.

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    Marek Grabowski and Ewa Niedzialkowska have contributed equally to this work.

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    Grabowski, M., Niedzialkowska, E., Zimmerman, M.D. et al. The impact of structural genomics: the first quindecennial. J Struct Funct Genomics 17, 1–16 (2016). https://doi.org/10.1007/s10969-016-9201-5

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