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Solution NMR structures of homeodomains from human proteins ALX4, ZHX1, and CASP8AP2 contribute to the structural coverage of the Human Cancer Protein Interaction Network

  • Published:
Journal of Structural and Functional Genomics

Abstract

High-quality solution NMR structures of three homeodomains from human proteins ALX4, ZHX1 and CASP8AP2 were solved. These domains were chosen as targets of a biomedical theme project pursued by the Northeast Structural Genomics Consortium. This project focuses on increasing the structural coverage of human proteins associated with cancer.

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Abbreviations

ALX4:

Aristaless-like 4

ZHX1:

Zinc fingers and homeoboxes protein 1

CASP8AP2:

Caspase 8 associated protein 2

Al:

Aristaless

HCPIN:

Human Cancer Pathway Interaction Network

DSS:

4,4-Dimethyl-4-silapentane-1-sulfonate sodium salt

DTT:

Dithiothreitol

NESG:

Northeast Structural Genomics Consortium

NOE:

Nuclear Overhauser effect

PDB:

Protein Data Bank

RMSD:

Root mean square deviation

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Acknowledgments

This work was supported by the National Institutes of Health, Grant Number: U54 GM094597 (T.S. and G.T.M.).

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Authors and Affiliations

  1. Department of Chemistry, The State University of New York at Buffalo and Northeast Structural Genomics Consortium, Buffalo, NY, 14260, USA

    Xianzhong Xu, Surya V. S. R. K. Pulavarti, Alexander Eletsky & Thomas Szyperski

  2. Center of Advanced Biotechnology and Medicine and Department of Molecular Biology and Biochemistry, Rutgers, The State University of New Jersey and Northeast Structural Genomics Consortium, Piscataway, NJ, 08854, USA

    Yuanpeng Janet Huang, Thomas B. Acton, Rong Xiao, John K. Everett & Gaetano T. Montelione

  3. Department of Biochemistry and Molecular Biology, Robert Wood Johnson Medical School, UMDNJ, Piscataway, NJ, 08854, USA

    Gaetano T. Montelione

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  1. Xianzhong Xu
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  2. Surya V. S. R. K. Pulavarti
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  3. Alexander Eletsky
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Correspondence to Thomas Szyperski.

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Xu, X., Pulavarti, S.V.S.R.K., Eletsky, A. et al. Solution NMR structures of homeodomains from human proteins ALX4, ZHX1, and CASP8AP2 contribute to the structural coverage of the Human Cancer Protein Interaction Network. J Struct Funct Genomics 15, 201–207 (2014). https://doi.org/10.1007/s10969-014-9184-z

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