Solution NMR structure of the helicase associated domain BVU_0683(627–691) from Bacteroides vulgatus provides first structural coverage for protein domain family PF03457 and indicates domain binding to DNA

  • Jeffrey L. Mills
  • Thomas B. Acton
  • Rong Xiao
  • John K. Everett
  • Gaetano T. Montelione
  • Thomas SzyperskiEmail author


A high-quality NMR structure of the helicase associated (HA) domain comprising residues 627–691 of the 753-residue protein BVU_0683 from Bacteroides vulgatus exhibits an all α-helical fold. The structure presented here is the first representative for the large protein domain family PF03457 (currently 742 members) of HA domains. Comparison with structurally similar proteins supports the hypothesis that HA domains bind to DNA and that binding specificity varies greatly within the family of HA domains constituting PF03457.


A6KY75_BACV8 BVU_0683 PF03457 Helicase associated domain Structural genomics SANT domain 



Deoxyribonucleic acid


4,4-dimethyl-4-silapentane-1-sulfonate sodium salt




Helicase associated


Imitation switch


2-(N-morpholino)ethanesulfonic acid


Northeast structural genomics consortium


Nuclear overhauser effect


Protein data bank


Root mean square deviation





We thank R. Belote, C. Ciccosanti, K. Hamilton, and G.V.T. Swapna for helpful discussions and technical support. This work was supported by the National Institutes of Health, grant number: U54 GM094597 (T.S. and G.T.M.).

Supplementary material

10969_2012_9148_MOESM1_ESM.doc (3.7 mb)
Supplementary material 1 (DOC 3,789 kb). The online version of this article contains supplementary material, which is available to authorized users


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Copyright information

© Springer Science+Business Media Dordrecht 2012

Authors and Affiliations

  • Jeffrey L. Mills
    • 1
  • Thomas B. Acton
    • 2
  • Rong Xiao
    • 2
  • John K. Everett
    • 2
  • Gaetano T. Montelione
    • 2
    • 3
  • Thomas Szyperski
    • 1
    Email author
  1. 1.Department of ChemistryThe State University of New York at Buffalo, and Northeast Structural Genomics ConsortiumBuffaloUSA
  2. 2.Center of Advanced Biotechnology and Medicine and Department of Molecular Biology and Biochemistry, RutgersThe State University of New Jersey and Northeast Structural Genomics ConsortiumPiscatawayUSA
  3. 3.Department of Biochemistry, Robert Wood Johnson Medical SchoolUMDNJPiscatawayUSA

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