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X-Ray crystal structure of GarR—tartronate semialdehyde reductase from Salmonella typhimurium

  • Published:
Journal of Structural and Functional Genomics

Abstract

Tartronate semialdehyde reductases (TSRs), also known as 2-hydroxy-3-oxopropionate reductases, catalyze the reduction of tartronate semialdehyde using NAD as cofactor in the final stage of d-glycerate biosynthesis. These enzymes belong to family of structurally and mechanically related β-hydroxyacid dehydrogenases which differ in substrate specificity and catalyze reactions in specific metabolic pathways. Here, we present the crystal structure of GarR a TSR from Salmonella typhimurium determined by the single-wavelength anomalous diffraction method and refined to 1.65 Å resolution. The active site of the enzyme contains l-tartrate which most likely mimics a position of a glycerate which is a product of the enzyme reaction. The analysis of the TSR structure shows also a putative NADPH binding site in the enzyme.

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Acknowledgments

We wish to thank all members of the Structural Biology Center at Argonne National Laboratory for their help in conducting experiments. This work was supported by National Institutes of Health Grant GM62414, GM074942 and by the US Department of Energy, Office of Biological and Environmental Research, under contract DE-AC02-06CH11357.

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Authors and Affiliations

  1. Biosciences Division, Midwest Center for Structural Genomics, Argonne National Laboratory, 9700 South Cass Ave., Bldg 202, Argonne, IL, 60439, USA

    J. Osipiuk, M. Zhou, S. Moy, F. Collart & A. Joachimiak

  2. Biosciences Division, Structural Biology Center, Argonne National Laboratory, 9700 South Cass Ave., Bldg 202, Argonne, IL, 60439, USA

    A. Joachimiak

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  1. J. Osipiuk
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  2. M. Zhou
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  3. S. Moy
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  4. F. Collart
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  5. A. Joachimiak
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Correspondence to A. Joachimiak.

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The submitted manuscript has been created by UChicago Argonne, LLC, Operator of Argonne National Laboratory (“Argonne”). Argonne, a US Department of Energy Office of Science laboratory, is operated under Contract No. DE-AC02-06CH11357. The US Government retains for itself, and others acting on its behalf, a paid-up nonexclusive, irrevocable worldwide license in said article to reproduce, prepare derivative works, distribute copies to the public, and perform publicly and display publicly, by or on behalf of the Government.

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Osipiuk, J., Zhou, M., Moy, S. et al. X-Ray crystal structure of GarR—tartronate semialdehyde reductase from Salmonella typhimurium . J Struct Funct Genomics 10, 249–253 (2009). https://doi.org/10.1007/s10969-009-9059-x

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  • DOI: https://doi.org/10.1007/s10969-009-9059-x

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