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New approach for in-house production of [68Ga]Ga-NOTA-UBI(29–41) using a titanium dioxide column-based generator

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Abstract

Efforts have been dedicated to distinguishing between infections and sterile inflammations. This study presents a novel approach for in-house synthesis of [68Ga]Ga-NOTA-UBI(29–41) employing a titanium dioxide column-based 68Ga-generator. The optimized method yielded the radiopeptide with high radiochemical purity (> 99%) and stability (up to 90 min). [68Ga]Ga-NOTA-UBI(29–41) demonstrates hydrophilic characteristics (Log P = − 3.57 ± 0.20) and a binding to serum proteins of ~ 60%. The affinity of [68Ga]Ga-NOTA-UBI(29–41) to bacteria was directly proportional to the increasing quantity of bacterial cells. Consequently, this method generates [68Ga]Ga-NOTA-UBI(29–41) with remarkable physicochemical characteristics and high binding affinity to bacterial cells, rendering it suitable for clinical applications.

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Data availability

The authors confirm that the data supporting the findings of this study are available within the article. Raw data that support the findings of this study are available from the corresponding authors, upon reasonable request.

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Acknowledgements

The authors would like to thank the Departamento de Medicina Nuclear and the Centro de Experimentação e Treinamento em Cirurgia (CETEC) of the Hospital Israelita Albert Einstein and the Santa Casa de Sao Paulo School of Medical Sciences. CRPS also thanks the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) for the fellowship.

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Authors and Affiliations

  1. Department of Physiological Sciences, Santa Casa de Sao Paulo School of Medical Sciences, Sao Paulo, SP, Brazil

    Caiubi Rodrigues de Paula Santos, Leonardo Lima Fuscaldi, Ana Claudia Ranucci Durante & Luciana Malavolta

  2. Hospital Israelita Albert Einstein, Sao Paulo, SP, Brazil

    Caiubi Rodrigues de Paula Santos, Ana Claudia Ranucci Durante & Marycel Figols de Barboza

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  1. Caiubi Rodrigues de Paula Santos
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  2. Leonardo Lima Fuscaldi
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Correspondence to Leonardo Lima Fuscaldi or Luciana Malavolta.

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Santos, C.R.d., Fuscaldi, L.L., Durante, A.C.R. et al. New approach for in-house production of [68Ga]Ga-NOTA-UBI(29–41) using a titanium dioxide column-based generator. J Radioanal Nucl Chem 333, 1253–1262 (2024). https://doi.org/10.1007/s10967-024-09377-x

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