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Synthesis and in vitro evaluation of 99mTc radiolabeled lapatinib (LPT) and its PLGA formulation

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Abstract

Lapatinib-(LPT) is a dual tyrosine kinase inhibitor that has important effects on the targeted therapy of various tumours. The current study is aimed to use a novel tracer strategy including Technetium-99 m-(99mTc) radiolabeled LPT and its poly(lactic-co-glycolic acid)-(PLGA) encapsulated formulation-(LPT-PLGA). In vitro bioaffinity of the [99mTc]Tc-LPT and [99mTc]Tc-LPT-PLGA was evaluated by using cell culture methods on human breast adenocarcinoma (MDA-MB-231), cervix adenocarcinoma and human ovarian (MDAH-2774) cancer cell lines. Radiochemical yield were over 95% and both compounds were examined high incorporation values on HeLa and MDAH cell lines. Current results may contribute to develope as the encapculated agents including radiotracer.

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Acknowledgements

Current work is supported by Ege University Scientific Research Projects Coordination (contract no 2017-TIP-044). We would like to thank Pál Ferenc to check for grammatical and language mistakes. The authors also thank Nese Kavcar, Fatmagul Gedik, Oyku Madenci for the technical assistance during the assays.

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Authors and Affiliations

  1. Department of Gynaecologic Oncology, Medical Faculty Hospital, Mersin University, Mersin, Turkey

    Sevki Goksun Gokulu

  2. Department of Nuclear Applications, Institute of Nuclear Sciences Ege University, 35100, Izmir, Turkey

    Kadriye Busra Karatay, Ayfer Yurt Kilcar & Fazilet Zumrut Biber Muftuler

  3. Department of Obstetrics and Gynaecology, Faculty of Medicine, Selçuk University, Konya, Turkey

    Ahmet Bilgi

  4. Department of Medical Physics, Institute of Health Sciences, Dokuz Eylul University, Izmir, Turkey

    Cansu Kayas

  5. Department of Obstetrics and Gynaecology, Faculty of Medicine, Ege University, Izmir, Turkey

    Nuri Yildirim, Mustafa Cosan Terek & Levent Akman

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  1. Sevki Goksun Gokulu
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Correspondence to Fazilet Zumrut Biber Muftuler.

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Gokulu, S.G., Karatay, K.B., Bilgi, A. et al. Synthesis and in vitro evaluation of 99mTc radiolabeled lapatinib (LPT) and its PLGA formulation. J Radioanal Nucl Chem 333, 665–672 (2024). https://doi.org/10.1007/s10967-023-09292-7

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  • DOI: https://doi.org/10.1007/s10967-023-09292-7

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