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A novel SPECT/MRI bimodal imaging probe: 99mTc-DPAPA-Fe3O4 nanoconjugate

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Abstract

This study aimed to create a novel bimodal probe. For this purpose, cubic iron oxide nanoparticles (C-Fe3O4 NPs) conjugated with an EDTMP derivative called DPAPA thought to be a targeting tool for prostate cancer and bone metastases were studied. NPs were synthesized and conjugated with DPAPA, and then characterization studies were carried out. The particles were demonstrated to be cubic shaped, with an average size of 68.55 ± 1 0.03 (n = 3) nm. Then the nanoconjugates were radiolabeled with 99mTc. In vitro cellular affinities were conducted using PC-3, RWPE-1, LNCaP, K562, and Saos-2 cell lines. In conclusion, radio-nanoconjugates have the potential for bimodal MRI/SPECT imaging.

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Abbreviations

C-Fe3O4 :

Cubic iron oxide

C-Fe3O4-SiO2 :

Cubic iron oxide coated with TEOS

C-Fe3O4-SiO2-HPG:

C-Fe3O4-SiO2 modified with hyperbranched polyglycerol (HPG)

C-Fe3O4-SiO2-HPG-NH2 :

Coupled ethylene diamine with C-Fe3O4-SiO2-HPG

C-Fe3O4-SiO2-HPG-NH2-DPAPA:

DPAPA conjugated with C-Fe3O4-SiO2-HPG-NH2 (NC)

99mTc-DPAPA-Fe3O4 :

Radiolabelled-nanocaonjugate (99mTc-C-Fe3O4-SiO2-HPG-NH2-DPAPA)

DPAPA:

(S)-2,5-Bis(bis(phosphonomethyl)amino) pentanoic acid

NPs:

Nanoparticles

NC:

Nanoconjugate

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Acknowledgements

This work was supported financially by TUBITAK (The Scientific and Technological Research Council of Turkey) within the scope of the Bilateral Cooperation Project No. 218S749. The authors thank TUBITAK for the support. Volkan Yasakçı was also supported through the 100/2000 CoHE Doctorate Scholarship and Turkish Higher Education Council (YÖK) 100/2000 Scholarship. The authors thank the Turkish Higher Education Council (YÖK) and 100/2000 CoHE Ph.D. for their support. Dr. Omer Aras was partially supported through the NIH/NCI Cancer Support Grant P30 CA008748. The work was partially presented at the International Conference on Clinical PET-CT and Molecular Imaging in the Era of Theranostics, IPET-2020, IAEA-CN-285/52, and the International Nuclear Sciences and Technologies Conference (INSTEC-22).

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Authors and Affiliations

  1. Institute of Nuclear Sciences, Department of Nuclear Applications, Ege University, 35100, Bornova, Izmir, Turkey

    Volkan Yasakçı, Elif Tutun, Kadriye Buşra Karatay & Perihan Ünak

  2. Department of Radiology, Memorial Sloan Kettering Cancer Center, New York, NY, 10065, USA

    Ömer Aras

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  1. Volkan Yasakçı
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Correspondence to Volkan Yasakçı.

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Yasakçı, V., Tutun, E., Karatay, K.B. et al. A novel SPECT/MRI bimodal imaging probe: 99mTc-DPAPA-Fe3O4 nanoconjugate. J Radioanal Nucl Chem 332, 4651–4666 (2023). https://doi.org/10.1007/s10967-023-08875-8

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