Abstract
Immunoassays employed to measure C-peptide in rat serum neither have high sensitivity nor good range. Hence, the aim of this work was to develop a sensitive and specific user friendly immunoradiometric assay (IRMA) utilizing in-house magnetizable cellulose particles for quantification of C-peptide in rat serum. For development of IRMA, detector antibody (M1*) was prepared by chloramine-T method using 125I and capture antibody (SM2) was prepared by carbonyldiimidazole (CDI) activation method. The matched pair M1*-SM2 yielded an assay with adequate sensitivity (0.24 ng/ml) and range (0–20 ng/ml). C-peptide concentrations in rat serum samples (n = 30) analyzed using the developed assay ranged between 0.3 and 0.54 ng/ml. The reported C-peptide range can be used as a reference in impending diabetic research carried out using rat models.
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Acknowledgements
The author Manupriya B R is grateful to the University Grant Commission Basic Scientific Research (UGC-BSR) fellowship for extending financial support to carry out this work. The author is grateful to Dr. Karunakara Hedge, Department of Pharmacology, Srinivasa College of pharmacy, Mangaluru, India- 574143 for providing the animal house and assistance in blood collection from rats.
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Manupriya, B.R., Paradkar, S., Ghodke, T.S. et al. Development of a magnetizable cellulose particle-based immunoradiometric assay for quantification of C-peptide in rat serum. J Radioanal Nucl Chem 332, 517–525 (2023). https://doi.org/10.1007/s10967-023-08796-6
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DOI: https://doi.org/10.1007/s10967-023-08796-6