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Development of a magnetizable cellulose particle-based immunoradiometric assay for quantification of C-peptide in rat serum

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Abstract

Immunoassays employed to measure C-peptide in rat serum neither have high sensitivity nor good range. Hence, the aim of this work was to develop a sensitive and specific user friendly immunoradiometric assay (IRMA) utilizing in-house magnetizable cellulose particles for quantification of C-peptide in rat serum. For development of IRMA, detector antibody (M1*) was prepared by chloramine-T method using 125I and capture antibody (SM2) was prepared by carbonyldiimidazole (CDI) activation method. The matched pair M1*-SM2 yielded an assay with adequate sensitivity (0.24 ng/ml) and range (0–20 ng/ml). C-peptide concentrations in rat serum samples (n = 30) analyzed using the developed assay ranged between 0.3 and 0.54 ng/ml. The reported C-peptide range can be used as a reference in impending diabetic research carried out using rat models.

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Acknowledgements

The author Manupriya B R is grateful to the University Grant Commission Basic Scientific Research (UGC-BSR) fellowship for extending financial support to carry out this work. The author is grateful to Dr. Karunakara Hedge, Department of Pharmacology, Srinivasa College of pharmacy, Mangaluru, India- 574143 for providing the animal house and assistance in blood collection from rats.

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Authors and Affiliations

  1. Department of Applied Zoology, Mangalore University, Mangaluru, 574199, India

    B. R. Manupriya & K. Bhasker Shenoy

  2. Centre for Application of Radioisotopes and Radiation Technology (CARRT), Mangalore University, Mangaluru, 574199, India

    Tanhaji Sandu Ghodke & N. Karunakara

  3. Radiopharmaceuticals Programme, Board of Radiation and Isotope Technology (BRIT), Vashi Complex, Sector 20, Navi Mumbai, 400703, India

    Shalaka Paradkar & Vijay Kadwad

Authors
  1. B. R. Manupriya
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  2. Shalaka Paradkar
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  3. Tanhaji Sandu Ghodke
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  4. Vijay Kadwad
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  5. N. Karunakara
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  6. K. Bhasker Shenoy
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Correspondence to K. Bhasker Shenoy.

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Manupriya, B.R., Paradkar, S., Ghodke, T.S. et al. Development of a magnetizable cellulose particle-based immunoradiometric assay for quantification of C-peptide in rat serum. J Radioanal Nucl Chem 332, 517–525 (2023). https://doi.org/10.1007/s10967-023-08796-6

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  • DOI: https://doi.org/10.1007/s10967-023-08796-6

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