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Preliminary study on the improvement of identifying effect of Tc-99 m labeled C595 monoclonal antibody in MUC1 imaging of ovarian cancer

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Abstract

MUC1 imaging helps to guide the corresponding biological therapy of ovarian cancer. Herein, C595 antibody was cleaved to fab fragment, and then coupled with NHS-DTPA. The imaging effect using 99mTc-C595-Fab was evaluated on OVCAR-3 cells derived xenograft models. In results, the cleaved antibody did not affect the Tc-99 m labeling effect and in vitro stability. SPECT imaging proved the decreased accumulation of fragment in liver, together with the improved targeting efficiency in tumor, realizing the faster tumor targeting and higher tumor/liver ratio. Conclusively, C595 fragment realized the more efficient SPECT imaging of ovarian cancer.

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Acknowledgements

This research was funded by “Discipline Construction Climbing 234 Plan” of Changhai Hospital of Naval Medical University (Second Military Medical University) (2019YPT002) and Science and Technology Support Project in Biomedical Field of “Science and Technology Innovation Action Plan” of Shanghai Science and Technology Commission (21S21902100).

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Authors and Affiliations

  1. Family Planning Branch, Tangshan Maternity and Child Healthcare Hospital, Tangshan, 063000, Hebei, China

    Yan Zhang & Mei Zhang

  2. Department of Gynaecology and Obstetrics, Tangshan Maternity and Child Healthcare Hospital, Tangshan, 063000, Hebei, China

    Yuzhen Feng

  3. Department of Nephrology, Changhai Hospital, Naval Medical University, Shanghai, 200433, China

    Deliang Kong

  4. School of Medicine, Shanghai University, Shanghai, 200444, China

    Liyan Xiong

  5. Department of Nuclear Medicine, Shanghai Changhai Hospital, Shanghai, 200433, China

    Qinqin Yang

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  1. Yan Zhang
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  3. Mei Zhang
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  4. Deliang Kong
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Correspondence to Deliang Kong, Liyan Xiong or Qinqin Yang.

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Zhang, Y., Feng, Y., Zhang, M. et al. Preliminary study on the improvement of identifying effect of Tc-99 m labeled C595 monoclonal antibody in MUC1 imaging of ovarian cancer. J Radioanal Nucl Chem 331, 4189–4195 (2022). https://doi.org/10.1007/s10967-022-08478-9

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  • DOI: https://doi.org/10.1007/s10967-022-08478-9

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