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Comparative transcriptome analysis providing inhibitory mechanism of lung cancer A549 cells by radioactive 125I seed

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Abstract

In this study, A549 cells treated by radioactive 125I seed were sequenced using Illumina HiSeq PE150, and then the transcriptome was analyzed using bioinformatics methods. Gene ontology classification analysis showed that differentially expressed genes (DEGs) belonged to 10 categories, and low-dose group, medium-dose group and high-dose group 125I treatment obtained 440 DEGs, 4275 DEGs and 2850 DEGs, respectively. Additionally, DEGs were involved in tumor-related pathways, endoplasmic reticulum protein processing and cell cycle pathways. Therefore, radioactive 125I seed can significantly inhibit the growth of lung cancer, which provide a theoretical basis for the clinical 125I seed to effectively treat lung cancer.

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Acknowledgements

The program was supported by National Natural Science Foundation of China (21876179, 22142007 and 22006001), Natural Science Foundation of Anhui Province (1808085QA13 and 2008085MC65), China Postdoctoral Science Foundation (2020T130117ZX, 2020M671914 and 2020B470) and Natural Science Foundation of Anhui Higher Education Institutions (KJ2017A935).

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Authors and Affiliations

  1. Anhui Province Key Laboratory of Medical Physics and Technology, Institute of Health & Medical Technology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, 230031, People’s Republic of China

    Fang Liu, Jie Bao, Jinwu Chen & Wencheng Song

  2. School of Life Science, Anhui University, Hefei, 230036, People’s Republic of China

    Fang Liu

  3. Hefei Cancer Hospital, Chinese Academy of Sciences, Hefei, 230031, People’s Republic of China

    Jie Bao & Wencheng Song

  4. Hefei Normal University, Hefei, Anhui, 230061, People’s Republic of China

    Jinwu Chen

  5. Collaborative Innovation Center of Radiation Medicine of Jiangsu Higher Education Institutions and School for Radiological and Interdisciplinary Sciences, Soochow University, 215123, Suzhou, People’s Republic of China

    Wencheng Song

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  1. Fang Liu
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Correspondence to Jinwu Chen or Wencheng Song.

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Liu, F., Bao, J., Chen, J. et al. Comparative transcriptome analysis providing inhibitory mechanism of lung cancer A549 cells by radioactive 125I seed. J Radioanal Nucl Chem 330, 821–831 (2021). https://doi.org/10.1007/s10967-021-07984-6

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